厚朴酚通过自噬途径促进肺癌细胞死亡

目的:研究厚朴酚诱导人非小细胞性肺癌H460细胞的死亡作用并探讨其抗癌作用机制。方法:采用MTT法检测厚朴酚对H460细胞的生长抑制作用,TdT介导dUTP缺口末端标记法(TdT-medi-ated dUTP Nick-End Labeling,TUNEL)法和乳酸脱氢酶(L-Lactate Dehydrogenase,LDH)活力检测法检测细胞凋亡及坏死率,monodansyl-cadaverine(MDC)荧光染色法标记检测细胞自噬情况。结果:厚朴酚可时间依赖性抑制H460肺癌细胞的生长,荧光显微镜下观察厚朴酚作用24h后的细胞出现大量空泡;乳酸脱氢酶活力检测,厚朴酚处理48h后细胞坏死;TUNEL法检测发现,高浓度处理组的细胞很少发生凋亡。结论:厚朴酚主要通过细胞自噬途径而非凋亡途径诱导H460肺癌细胞的死亡。

Magnolol-induced Lung Cancer Cells Death via Autophagy

Objective： To explore the anti-tumor mechanism of Magnolol induced H460 cells death.Methods： MTT assay was used to test the effects of Magnolol to inhibit the growth of H460 cells;TUNEL assay and LDH Activity assays were used to prove the apoptotic and death ratios;MDC staining assay was used to label the vacuoles in H460 cells.Results： Magnolol could inhibit the growth of H460 cells in time-dependent.Administered by Magnolol 48h later,H460 cells had necrosis.Few apoptotic bodies were found in H460 cells of high concentrations of Magnolol in TUNEL assay.There were some vacuoles in H460 cells in MDC assay,which showed the occurrence of autophagy.Conclusion： Magnolol induced lung cancer H460 cells death via autophagy,but not apoptotic pathway.