微透析探针在体透析时间对氨基酸回收率的影响

目的：探讨在体透析不同时间后微透析探针的氨基酸体外回收率的变化。方法：采用微透析系统对使用前后的探针进行氨基酸标准液的体外透析，氨基酸标准液和探针透析液则用柱前衍生法配合高效液相色谱荧光检测器进行检测。结果：用再生纤维素膜制作的微透析探针在使用不同时间之后，天冬氨酸（Asp）、谷氨酸（Glu）和γ-氨基丁酸（GABA）体外回收率不完全相同（Asp：F=19．669，P=0．000；Glu：F=103．955，P=0．000；GABA：F=3．454，P=0．040）；牛磺酸（Tau）无显著性差异（F=2．001，P=0．152）。在使用6h后，Asp、Glu、Tau和GABA的回收率剩余百分率（RRP）分别为64．34％、67．36％、103．11％和98．23％，其中Asp、Glu有显著性下降（Asp：P〈0．01，Glu：P〈0．05）。在使用12h后，Asp、Glu、Tau和GABA的RRP分别为43．44％、24．42％、77．45％和67．36％，Asp、Glu和GABA有显著性下降（Asp：P〈0．001，Glu：P〈0．001，GABA：P〈0．05）。在使用24h后，Asp、Glu、GABA的RRP分别为36．26％、12．24％、89．48％和71．35％，Asp、Glu和GABA有显著性下降（Asp：P〈0．0001，Glu：P〈0．0001，GABA：P〈0．05）。结论：在体透析可引起氨基酸回收率的下降，透析时间越长，回收率下降越大。故在进行脑微透析实验时，探针的在体使用时间不宜过长；为了提高所得数据的可靠性，应对探针的回收率进行适当校正。

Effect of dialysis time in vivo on recovery of amino acids for microdialysis probe

Objective: To investigate the variety of vitro recovery of amino acids for microdialysis probe after different dialysis time in vivo.Methods: Probes were dialyzed in the amino acids standard solutions with microdialysis system,amino acid standard solutions and the microdialysate of probe were detected by the method of precolumn derivation with HPLC-RF.Results: After using different time of probe made by regenerated cellulose membrane,the vitro recoveries of Asp,Glu and GABA were not completely same(Asp:F=19.669,P=0.000;Glu:F=103.955,P=0.000;GABA:F=3.454,P=0.040);while the vitro recovery of Tau had no obvious difference(F=2.001,P=0.152).After using 6 h in vivo,recovery remain percentage(RRP) of Asp,Glu,Tau and GABA was 64.34%,67.36%,103.11% and 98.23%,respectively,the recoveries of Asp,Glu decreased obviously(Asp:P< 0.01,Glu:P<0.05).After using 12 h in vivo,the RRP of Asp,Glu,Tau and GABA was 43.44%,24.42%,77.45% and 67.36%,respectively,the recoveries of Asp,Glu and GABA decreased obviously(Asp:P<0.001,Glu:P<0.001,GABA:P<0.05).After using 24 h in vivo,the RRP of Asp,Glu,Tau and GABA was 36.26%,12.24%,89.48% and 71.35%,respectively,the recoveries of Asp,Glu,GABA decreased obviously(Asp:P<0.0001,Glu:P<0.0001,GABA:P<0.01).Conclusions: Dialysis in vivo could lead to the decline of recovery of probe,the decline is more obvious after longer dialysis.So when making brain dialysis experiments,the use time of probe should not be too long.To improve the validity of data,some calibration should be made on the recoveries of probe.