凝血酶诱导人胚胎肾系膜细胞增殖和PAI-1表达的研究

目的 :探讨凝血酶对人胚胎肾系膜细胞增殖和纤溶酶原激活物抑制物 1 (PAI 1 )表达的影响。方法 :采用噻唑蓝 (MTT)掺入法、Northern杂交和纤维蛋白平板法分别检测不同浓度凝血酶刺激下人胚胎肾小球系膜细胞增殖、PAI 1mRNA表达和PAI 1蛋白质活性的变化 ,并同时用人工水蛭素进行阻断实验。结果 :凝血酶呈浓度依赖性的方式促进人胚胎肾小球系膜细胞增殖、PAI 1mRNA表达和PAI 1蛋白质活性 ,水蛭素能特异地阻断凝血酶的作用。结论 :凝血酶可通过促进系膜细胞增殖、PAI 1的表达和提高其活性而导致肾脏损伤

Effect of thrombin on cell proliferation and plasminogen activator inhibitor type(PAI-1) expression in human embryonic glomerular mesangial cells

Objective: To explore the effect of thrombin on cell proliferation and plasminogen activator inhibitor Type 1(PAI 1) expression in human embryonic glomerular mesangial cells. Methods: Methyl tetragolium(MTT) incorporation, fibrin plate assay and Northern blotting hybridization were used to examine the mesangial cells proliferation, protein activity and mRNA expression of PAI 1 in cultured human embryonic mesangial cells induced by thrombin, respectively. Results: Thrombin enhanced glomerular mesangial cell proliferation, PAI 1 protein activity and mRNA expression in a dose dependent manner, which could be blocked by hirudin, a specific inhibitor of thrombin. Conclusion: Thrombin could enhance mesangial cell proliferation, and up regulate protein activity and mRNA expression of PAI 1 in cultured human embryonic glomerular mesangial cells. It may play an important role in the pathogenesis of glomerulosclerosis induced by thrombin. [