益坤精胶囊对去卵巢大鼠骨密度和骨组织中IL-6含量的影响

目的观察补肾中药益坤精胶囊对去卵巢大鼠骨密度的影响,并通过其对骨组织微环境中IL-6含量的影响以探讨其作用机制。方法通过切除大鼠双侧卵巢建立绝经后骨质疏松症模型。3月龄SD雌性大鼠50只,随机分为5组:假手术组、去卵巢组、尼尔雌醇组、中药高、低剂量组。去卵巢术后1 w,用灌胃法给服益坤精胶囊10w,以尼尔雌醇和生理盐水作为对照,观察各组大鼠骨密度及骨形态学的改变,并以RIA法检测各组大鼠骨组织培养液中IL6的含量。结果 与假手术组比较,去卵巢组骨密度显著下降(P<0.01),骨小梁稀疏断裂,骨组织中IL-6含量明显增高(P<0.01)。与去卵巢组比较,治疗组骨密度明显增高(P<0.01),但仍低于假手术组(P<0.05)。骨组织中IL-6含量显著下降(P<0.05)。骨形态学亦显著改善。结论益坤精胶囊能够抑制骨组织微环境中IL-6的产生,而起到抗骨质疏松的作用。

Effects of Yikunjing Capsules on bone mineral density and IL-6 levels in bone of ovariectomized rats

Objective To observe the effects of Yikunjing capsules on bone mineral density(BMD) and study its mechanism based on the changes of 1L-6 levels in bone of ovariectomized rats. Methods Adult ovariec-tomized rats were used in this study as the model of postmenopausal osteoporosis. 50 three-month-old female SD rats were randomly divided into 5 groups: sham operation group, ovariectomized(OVX) group, OVX with nilestriol group, OVX with Yikunjing groups in large and small dosages. Ten weeks after feeding with the medicines, the BMD and the IL-6 level in bone were detected, and histological changes of bone were observed. Results Compared with sham operation group, the general BMD in the OVX group decreased significantly (P < 0.01) while the IL-6 level in bone increased significantly (P < 0.01). The histology of bone revealed thin and irregular trabecu-lae. Compared with the OVX group, the general BMD in Yikunjing groups increased significantly (P < 0.01) while the IL-6 levels in bone decreased significantly (P < 0.01) . Conclusions Yikunjing capsules effectively prevent osteoporosis induced by ovariectomy in female rats, presumably by decreasing the IL-6 level in bone and inhibiting bone absorption.