| HPLC-ELSD法测定妇安栓剂中黄芪甲苷的含量 |
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| 引用本文： | 宋 敏. HPLC-ELSD法测定妇安栓剂中黄芪甲苷的含量[J]. 中国药房, 2014, 0(7): 647-648 |
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| 作者姓名： | 宋 敏 |
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| 作者单位： | 资阳市第一人民医院药剂科,四川资阳641300 |
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| 摘 要： | 目的:建立测定妇安栓剂中黄芪甲苷含量的方法。方法:采用高效液相色谱-蒸发光散射检测器法。色谱柱为Phenomenex C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(32∶68,V/V),流速为0.8 ml/min,柱温为30℃,蒸发温度为100℃,氮气流速为2.0 L/min。结果:黄芪甲苷进样量在2.6526.50μg范围内的对数值与峰面积积分值的对数值呈良好的线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD<3%;平均加样回收率为99.38%,RSD=1.60%(n=9)。结论:该方法简便、灵敏、重复性好,可用于妇安栓剂的质量控制。
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| 关 键 词： | 妇安栓剂 黄芪甲苷 高效液相色谱-蒸发光散射检测器法 含量测定 |
Content Determination of Astragaloside in Fuan Suppositories by HPLC-ELSD |
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| SONG Min. Content Determination of Astragaloside in Fuan Suppositories by HPLC-ELSD[J]. China Pharmacy, 2014, 0(7): 647-648 |
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| Authors: | SONG Min |
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| Affiliation: | SONG Min(Dept. of Pharmacy, Ziyang First People＇s Hospital, Sichuan Ziyang 641300, China) |
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| Abstract: | OBJECTIVE： To establish a method for the content determination of astragaloside in Fuan suppositories. METHODS： HPLC-ELSD was adopted. The determination was performed on Phenomenex C,8（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-water （32 ： 68, V/V） at the flow rate of 0.8 ml/min. The column temperature was set at 30 ℃, and evaporation temperature was 100 ℃. The flow rate of nitrogen was 2.0 L/min. RESULTS： The linear range of astragaloside was 2.65-26.50 μg（r=0.999 9） ; RSDs of precision, stability and reproducibility tests were lower than 3% ; average recovery was 99.38% （RSD= 1.60%, n=9）. CONCLUSIONS： The method is simple, sensitive and reproducibility. It can be used for the quality control for Fuan suppositories. KEYWORDS Fuan suppositories; Astragaloside; HPLC-ELSD; Content determination |
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