EBV转化淋巴细胞与EBV相关细胞核基质蛋白分析

［目的］研究脐淋巴细胞与ＥＢＶ转化淋巴细胞（ＬＣＩｓ），ＥＢＶ相关细胞核基质蛋白（ＮＭＰｓ）之间的关系，分析鉴定ＥＢＶ感染细胞后的ＮＭＰｓ特异性改变，探讨ＮＭＰｓ与ＥＢＶ转化及肿瘤形成的关系。［方法］ＥＢＶ转化淋巴细胞；改良方法提取ＮＭＰｓ；制备ＮＭＰｓ免疫小鼠血清；ＳＤＳ－ＰＡＧＥ电泳及双波长蛋白薄层扫描方法分析ＮＭＰｓ；免疫蛋白印迹分析ＮＭＰｓ与抗ＮＭＰｓ的阳性血清之间的反应性。［结果］获得ＥＢＶ转化淋巴细胞。获得五种细胞ＮＭＰｓ。各细胞间分子量相同的蛋白约占各自细胞组分的５２．１％～５９．６％。脐淋巴细胞３０ＫＤ～４０ＫＤ蛋白较多，而ＬＣＬｓ和ＥＢＶ相关淋巴细胞中，高分子量的蛋白比较多。－６４ＫＤ ＮＭＰ特异地存在于ＬＣＬｓ及ＥＢＶ相关细胞中。ＮＰＣ病人血清中能够检出抗ＬＣＬｓ的ＮＭＰｓ抗体。［结论］ＬｅｕＬｅｕ－ＯＭｅ和环孢菌素Ａ结合使用，可促进ＥＢＶ转化。低分化的脐淋巴细胞比高分化ＬＣＬｓ有更稳定的核基质网架结构。推测相同分子量ＮＭＰｓ构成核基质的结构成分；ＥＢＶ感染细胞后，ＮＭＰｓ有明显的变化。ＮＭＰｓ的特异性与ＥＢＶ感染高度相关；ＥＢＶ转化与ＮＰＣ的发生有一定关系。

Analysis of Nuclear Matrix Proteins of EBV-transformed Lymphoblastoid Cell lines and EBV-related Cell lines

Purpose]To study the relationship between cord blood lymphocytes and EBV-transformed lymphocytes (LCKs) or EBV-related cell nuclear matrix ptoteins(NMPs).To analyze the speific changes of NMPs after EBV-infection,and explore the possible tumorigenesis of NMPs or EBV-tranformation.[Metheds] NMPs was extracted and isolated from EBV-transfromed lymphocytes by using improved methods,and identitied by SDS-PAGE Gel Electrophoresis,dual wave length TLC Scanning and Westerm-Blot.[Results] Five types of NMPs were obtained form lymphocytes after EBV-transformation,with common molecular weight proteius making up about 52.1%~59.6% of all NMPs in foggrtrmy cell line.Cord lymphoblastoid Cells have more 30KD ~ 4OKD NMPs, and LCLs or EBV-related cells have more proteins with high molecular weight.One 64 KD NMPs were especially found in LCLs or EBV-related cells.Meanwhile,the antibodies of NMPs were idenfitied in the sera of NPC patients. [Conclusion] LeuLeu - OMe and Cyclosporine A can improve the efficiency and lessen the time of transformation of EBV;More stable nuclear matrix are existed in low-differentiated cord blood lymphocytes than in high-differentiated LCLs;Common molecular weigth proteins may constitute the nuclear fabrics of cells, and notable differences of NMPs can be found after EBV infecting cells.The speilist of NMPs are tightly related to the EBV- infection,and the EBV-transformation may be tumorigenesis of NPC.