| 胰高血糖素样肽1诱导小鼠胚胎干细胞E14分化为胰岛素表达细胞 |
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| 引用本文： | 杨川,程桦,王川,黎锋,魏菁. 胰高血糖素样肽1诱导小鼠胚胎干细胞E14分化为胰岛素表达细胞[J]. 中华内分泌代谢杂志, 2004, 20(3): 263-264 |
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| 作者姓名： | 杨川 程桦 王川 黎锋 魏菁 |
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| 作者单位： | 510120,广州,中山大学附属第二医院内分泌科 |
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| 摘 要： | 将小鼠胚胎干细胞株E14以高血糖素样肽 1(GLP 1)诱导 ,观察细胞形态 ,用原位杂交和RT PCR检测细胞内胰岛素mRNA的变化 ,免疫组化、流式细胞仪、放射免疫法验证细胞是否能分泌胰岛素样物质。发现E14细胞在GLP 1的作用下可以分化成表达胰岛素样物质的细胞
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| 关 键 词： | 胰高血糖素样肽1 胚胎干细胞 小鼠 E14细胞 胰岛素 |
| 修稿时间： | 2003-01-16 |
Glucagon-like peptide-1 induces mouse embryonic stem cells E14 to differentiate into insulin-expressing cells |
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| YANG Chuan,CHENG Hua,WANG Chuan,LI Feng,WEI Jing. Glucagon-like peptide-1 induces mouse embryonic stem cells E14 to differentiate into insulin-expressing cells[J]. Chinese Journal of Endocrinology and Metabolism, 2004, 20(3): 263-264 |
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| Authors: | YANG Chuan CHENG Hua WANG Chuan LI Feng WEI Jing |
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| Affiliation: | YANG Chuan,CHENG Hua,WANG Chuan,LI Feng,WEI Jing. Department of Endocrinology,The Second Hospital Affiliated to Sun Yat-sen University,Guangzhou 510120 |
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| Abstract: | Mouse embryonic stem cells E14 were co-cultured with various concentrations of glucagon-like peptide-1 (GLP-1) and cell morphological changes were observed. Insulin was detected by means of in situ hybridization, RT-PCR, immunocytochemistry, flow cytometer and radioimmunoassay. E14 cells induced by GLP-1 show the potential of differentiation to cells that express insulin-like substance. |
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| Keywords: | Glucagon-like peptide-1 Embryonic stem cell mouse E14 cells Insulin |
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