bFGF诱导的心肌微血管内皮细胞蛋白质组变化

目的:研究碱性成纤维细胞生长因子(bFGF)对培养的心肌微血管内皮细胞(MMEC)蛋白质表达变化的影响。方法:将培养的MMEC随机分为二组(n均=3):(1)bFGF诱导组(bFGF组):向培养瓶内加入终浓度10ng/ml的bFGF,置细胞培养箱内常氧培养24h。(2)对照组:常规37℃培养箱孵育。二组均提取细胞总蛋白质双向凝胶电泳后选取8个差异表达蛋白点进行胶内酶切、肽质量指纹图谱分析。结果:双向凝胶电泳可分离出约500±10个蛋白质。与对照组相比,bFGF组MMEC特异表达8种蛋白,上调1种蛋白表达,下调3种蛋白表达(bFGF组和对照组蛋白点的Volume值相差≥2.0倍)。结论:bFGF能诱导MMEC蛋白质差异表达。

Basic Fibroblast Growth Factor-induced Proteomics Profile in Myocardial Microvascular Endothelial Cells

Objective:To investigate basic fibroblast growth factor(bFGF)-induced proteomic profile in cultured myocardial microvascular endothelial cells(MMEC)from rats.Method:The cultured MMEC were randomly divided into control and bFGF groups(n=3).The bFGF group was incubated with bFGF(10 ng/mL).The control group was cultured at 37℃ in incubator.The whole protein extracts from MMEC were analyzed with two-dimensional polyacrylamide gel electrophoresis(2-DE).Eight differentially expressed proteins were identified by in-gel digestion and Mass Spectrum.Results:500 ±10 protein spots are resolved in cultured MMEC.Compared with control group,eight proteins expressed only in cultured MMEC treated with bFGF,one protein unregulated three proteins down regulated in MMEC treated with bFGF.Conclusion:bFGF treatment induces differential proteomics profile in cultured MMEC compared with control.