不同体外诱导时间对BMSCS成软骨分化的组织学特性影响的研究

目的探索体外不同时间的成软骨诱导,对骨髓间充质干细胞(BMSCs)体外成软骨分化的组织学特性的影响。方法以猪第2代BMSCs为种子细胞,以聚羟基乙酸/聚乳酸(PGA/PLA)为支架,体外构建直径12 mm,厚3 mm的圆盘状复合物,联合应用TGF-β1(10 ng/mL)、IGF-I(50 ng/mL)及地塞米松(40 ng/mL),体外分别诱导1周和4周,并以未经诱导的BMSCs和软骨细胞为对照,分析和比较不同诱导时间对BMSCs体外成软骨分化的影响。结果体外诱导4周的BMSCs-PGA/PLA复合物的组织学结构明显较只诱导1周的致密,软骨特异性基质(如蛋白多糖、Ⅱ型胶原等)表达水平明显高于诱导1周的。结论体外诱导时间对BMSCs软骨定向分化具有重要影响,延长诱导时间可明显促进复合物成熟并增加其软骨基质分泌。

The Effectiveness of Different Induction Time in Vitro on the Histological Characters of the Cartilaginous Tissue Conducted by Bone Marrow Stromal Cells

Objective To explore the effect of different induction time in vitro on the histological characters of the cartilaginous tissue conducted by bone marrow stromal cells（BMSCs）.Methods Porcine BMSCs of the second passage were injected into polyglycolic acid / polylactic acid discs.The biomaterial scaffold is 12 mm in diameter and 3 mm in thickness.The constructs were induced by a combination of TGF-β1（10 ng/mL）,IGF-I（50 ng/mL）,and dexamethasone（40 ng/mL） 1 week and 4 weeks respectively and were compared with control group（BMSCs group and cartilage cells group）.The relationship between the different induced time was analyzed and compared thoroughly.Results The histological structure of the groups induced 4 weeks is more solid than the group induced 1 week.Protein levels of cartilage special matrix（such as proteoglycan and Ⅱ type collagen et al） of the BMSCs-PGA/PLA construct induced 4 weeks in vitro detected with histological stain was superior to the groups induced 1 week.Conclusion The induction time is of great importance for BMSCs to develop into cartilage,and the longer of the inducing time,the stronger of the specific matrices expression.