| IL-6对人多发性骨髓瘤细胞鞘氨醇激酶的激活作用 |
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| 引用本文： | 孙慧燕,段海峰,贾向旭,李庆芳,刘玉和,吴祖泽,王立生.IL-6对人多发性骨髓瘤细胞鞘氨醇激酶的激活作用[J].军事医学科学院院刊,2004,28(2):130-132,135. |
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| 作者姓名： | 孙慧燕 段海峰 贾向旭 李庆芳 刘玉和 吴祖泽 王立生 |
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| 作者单位： | 1. 北华大学医学院,吉林,132001
2. 军事医学科学院放射医学研究所,北京,100850 |
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| 摘 要： | 目的：鞘氨醇激酶是细胞内合成磷酸鞘氨醇的激酶。该酶是细胞迁移、增殖及凋亡调节中发挥重要作用的信号分子。本研究拟确定鞘氨醇激酶在人多发性骨髓瘤细胞的表达及在IL-6信号途径中的作用。方法：采用RT-PCR方法鉴定了鞘氨醇激酶在骨髓瘤细胞的表达情况，通过鞘氨醇激酶活性测定确定IL-6对多发性骨髓瘤细胞鞘氨醇激酶的激活作用。结果：人多发性骨髓瘤细胞表达鞘氨醇激酶及S1P受体EDG1，3，5。IL-6通过PI-3K和MAPK激活鞘氨醇激酶。结论：在多发性骨髓瘤细胞中，鞘氨醇激酶参与IL-6的信号转导。鞘氨醇激酶有可能成为多发性骨髓瘤治疗的新靶点。
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| 关 键 词： | 鞘氨醇激酶 白细胞介素6 多发性骨髓瘤 |
| 文章编号： | 1000-5501(2004)02-0130-04 |
IL-6 activates sphingosine kinase in the human multiple myeloma cells |
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| SUN Hui-Yan ,DUAN Hai-Feng ,JIA Xiang-Xu ,LI Qing-Fang ,LIU Yu-He ,WU Chu-Tse ,WANG Li-Sheng.IL-6 activates sphingosine kinase in the human multiple myeloma cells[J].Bulletin of the Academy of Military Medical Sciences,2004,28(2):130-132,135. |
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| Authors: | SUN Hui-Yan DUAN Hai-Feng JIA Xiang-Xu LI Qing-Fang LIU Yu-He WU Chu-Tse WANG Li-Sheng |
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| Institution: | SUN Hui-Yan 2,DUAN Hai-Feng 1,JIA Xiang-Xu 1,LI Qing-Fang 1,LIU Yu-He 2,WU Chu-Tse 1,WANG Li-Sheng 1 |
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| Abstract: | Objective: To demonstrate the role of sphingosine kinase in the signal transduction of IL-6 in the multiple myeloma cells. Methods: RT-PCR was used to identify the expression of sphingosine kinase by SKO-007 cells. The cellular sphingosine kinase activity assay was employed to demonstrate the role of sphingosine kinase in IL-6 signal transduction. Results: SKO-007 cells expressed sphingosine kinase and the receptors of sphingosine 1-phosphate, endothelial differentiation gene 1 3,5. IL-6 activated sphingosine kinase in the human multiple myeloma cells via PI-3K and MAPK pathways. Conclusion: Sphingosine kinase is involved in the IL-6 signal transduction in the human multiple myeloma cells and is likely to be a therapeutic target. |
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| Keywords: | sphingosine kinase IL-6 multiple myeloma |
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