| pcDNA 3.1-AKR1B10真核表达载体的构建 |
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| 引用本文： | 杨少龙,徐文涛,石斌.pcDNA 3.1-AKR1B10真核表达载体的构建[J].现代诊断与治疗,2012,23(2):95-97. |
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| 作者姓名： | 杨少龙 徐文涛 石斌 |
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| 作者单位： | 郑州铁路职业技术学院,河南郑州,450052 |
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| 摘 要： | 从胰腺癌PaTu8988S细胞中提取总RNA,并克隆AKR1B10基因,构建pcDNA 3.1-AKR1B10真核表达载体。转化感受态大肠杆菌DH5α细胞,提质粒,酶切及测序鉴定,pcDNA 3.1-AKR1B10真核表达载体构建成功。
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| 关 键 词： | AKR1B10 pcDNA3.1 真核表达载体 |
Construction of Eukaryotic Expression Vector pcDNA 3.1-AKR1B10 |
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| YANG Shao-long
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XU Wen-tao
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SHI bin.Construction of Eukaryotic Expression Vector pcDNA 3.1-AKR1B10[J].Modern Diagnosis & Treatment,2012,23(2):95-97. |
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| Authors: | YANG Shao-long XU Wen-tao SHI bin |
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| Institution: | (Zhengzhou Railway Vocational ＆ Technical College,Zhengzhou 450052,China) |
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| Abstract: | Total RNA was extracted from pancreatic cancer PaTu8988S cell.AKR1B10 gene was cloned,and eukaryotic expression vector pcDNA 3.1-AKR1B10 was constructed.E.coli.DH5α cells were transformed into competent.Plasmids extraction,enzyme digestion and sequencing analysis were conducted.pcDNA 3.1-AKR1B10 eukaryotic expression vector was successfully constructed. |
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| Keywords: | AKR1B10 pcDNA 3 1 Eukaryotic expression vector |
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