大肠粘膜c-myc蛋白和增殖细胞核抗原表达的相关性研究

本文应用c-myc癌基因蛋白和增殖细胞核抗原(PCNA)免疫组织化学方法,研究了它们在正常大肠粘膜、结肠炎、腺瘤和腺癌中的表达。发现结肠炎和肿瘤c-myc蛋白和PCNA阳性率较正常粘膜高。在管状腺瘤、管状绒毛状腺瘤和绒毛状腺瘤,阳性率呈进行性增加,但没有统计学意义。c-myc蛋白和PCNA表达呈相关性。肿瘤中阳性细胞呈异质性,所以要得到可靠的研究结果应多点取材。

Correlation Study of c-myc Protein and Proliferating Cell Nuclear Antigen (PCNA) Expression in Colonic Mucosa

Two monoclonal antibodies, anti-c-myc protein and proliferating cell nuclear antigen (PCNA), were used in an immunohistoche-mical reaction to examine the colonic epithelium in normal mucosa, colitis, adenoma and carcinoma. In colitis and tumors the proportion of c-myc protein and PCNA-positive epithelial cells was higher than in normal. There exists a trend that the positive rates of c-myc protein and PCNA are progressively higher in the sequence of tubular, tubovillous and villous adenomas, al-though not significant (Pc-myc = 0. 5293, PPCNA = 0. 5450). There is a good correlation between c-myc protein and PCNA expression. The distribution of positive cells in the tumor is heteroge-nous, so more biopsies in a tumor must be studied to obtain reliable results of the tumor prolif-erative kinetics.