酪氨酸激酶Syk基因在乳腺癌组织中的表达及其临床意义

目的　探讨酪氨酸激酶Syk基因表达与乳腺癌生成及转移的关系 ,以及与雌激素受体(ER)、孕激素受体 (PR)、p5 3、HER2 /neu基因的关系。方法　用半定量逆转录聚合酶链反应检测 4 0例乳腺癌患者癌组织及癌旁正常乳腺组织以及 15例良性乳房纤维瘤组织中SykmRNA的表达 ,并用免疫组化方法检测 4 0例乳腺癌组织中ER、PR、p5 3、HER2 /neu的表达的情况。结果　所有正常乳腺组织都有Syk基因的表达 ,而 4 0例乳腺癌组织中只有 9例表达 ,正常乳腺与乳腺癌组织中Syk基因表达率差异有显著意义 (χ2 =4 7 4 ,P <0 0 5 ) ,且乳腺癌组织中SykmRNA含量明显低于正常乳腺组织 (t =3 4 1,P <0 0 5 )。有淋巴结转移的 18例乳腺癌组织中 ,1例有Syk基因表达 ,有淋巴结转移的乳腺癌SykmRNA的表达率和表达水平显著降低 (χ2 =3 77,P <0 0 5 ,t=2 74 ,P <0 0 5 )。SykmRNA表达与 p5 3基因的表达呈正相关。 结论　Syk基因的表达在抑制乳腺癌的生长及转移过程中可能起着重要的作用

Expression of tyrosine kinase Syk in breast cancer and their clinical significance

OBJECTIVE: To evaluate the effects of the Syk mRNA expression in human breast cancer on tumor growth and metastasis, and to study the correlation of expression of the Syk gene with ER, PR, p53 and HER2/neu. METHODS: Specimens from 40 breast cancer patients (tumor tissues, adjacent normal tissues), 15 fibroadenoma were detected for their expression of the Syk gene and level of Syk mRNA by semi-RT-PCR technique. Meanwhile, ER, PR, p53, HER2/neu were detected in 40 tumor tissues from breast cancer with immunohistochemical staining. RESULTS: All normal breast tissues were detected the expression of the Syk gene. Unlike normal breast tissue, 31 out of 40 breast cancer tissue did not show any detectable Syk mRNA expression, there were significant difference in two groups (chi(2) = 47.4, P < 0.05). The level of Syk mRNA in the primary breast cancer tissues were significantly lower than that in the adjacent non-cancerous breast tissues (t = 3.41, P < 0.05). Furthermore, only one breast cancer tissue in 18 patients with lymph node metastasis had the Syk mRNA expression, the rate and level of Syk mRNA expression in the patients with lymph node metastasis were lower than those without lymph node metastasis (chi(2) = 3.77, P < 0.05, t = 2.74, P < 0.05). Syk expression was correlated to p53 expression. CONCLUSION: The expression of the Syk gene may play an important role in suppressing growth and metastasis of breast cancer.