均匀设计法优化长春碱分子印迹聚合物的制备工艺

目的:优化长春碱分子印迹聚合物的制备工艺。方法:采用均匀设计法,考察因素包括功能单体甲基丙烯酸(MAA)的用量、交联剂乙二醇二甲基丙烯酸酯(EDMA)的用量以及致孔剂(溶剂),评价指标为对模板分子长春碱(VLB)的吸附率。结果:当VLB用量为0.1 mmol时,MAA和EDMA的用量分别为0.4 mmol、1.6 mmol,致孔剂为乙腈,在此条件下制备的长春碱分子印迹聚合物,对长春碱的吸附率为88.20%。并采用红外光谱、扫描电镜对聚合物结构进行表征。结论:用均匀设计法优化聚合反应的过程参数,方法可行,结果可靠,制备的聚合物对模板分子VLB表现出特异的亲和性。

Optimization of the Polymerization Conditions of Vinblastine Imprinted Polymer by Uniform Design

Objective:To optimize the polymerization conditions of vinblastine(VLB) imprinted polymer.Methods:The conditions were optimized by the method of uniform design.The major factors investigated included the amount of functional monomer(MAA) and the cross-linker(EDMA) and the progenic solvent(toluene or acetonitril).The adsorption rate of VLB on the solid-phase extraction(SPE) column packed with MIP was adopted as the response value.Results:The optimal conditions were MAA 0.4 mmol,EDMA 1.6 mmol and using acetonitrile as the solvent.Under the conditions,the VLB imprinted polymer was synthesized and the absorption rate of VLB was 88.20%.The characterizations of the optimal MIP were determined by IR spectrometry and scanning electron microscope(SEM) analysis.Conclusion:It is possible to furtherly improve the nature of the polymer by optimizing the polymerization parameters with uniform design.The polymer synthesized under the optimal conditions exhibited high affinity to the target molecule VLB.