半夏古法“汤洗”炮制的科学性分析

目的:评价经典名方中半夏“汤洗”炮制的科学性和可行性,为相关经典名方的开发提供参考.方法:基于古代“汤洗”炮制建立水洗半夏炮制方法,通过单因素试验优选工艺条件,测定炮制前后水分、灰分、浸出物,对其炮制前后质量变化、总酸含量(以琥珀酸计)以及高效液相色谱法(HPLC)指纹图谱进行比对,并进行家兔眼刺激试验,以评价水洗半夏...

Scientific Analysis of Processing of Pinelliae Rhizoma by Ancient Hot Water Washing Method

Objective: To evaluate the scientificity and feasibility of processing of Pinelliae Rhizoma by hot water washing(Tangxi),and to provide reference for the development of related famous classical formulas. Method: Processing method of Pinelliae Rhizoma washed by hot water was established based on ancient Tangxi processing method,and the process conditions were optimized by single factor tests. The weight,moisture, ash, extract, total acid(calculated by succinic acid) contents and high performance liquid chromatography(HPLC) fingerprint of Pinelliae Rhizoma were compared before and after processing. In addition,the rabbit eye irritation test was conducted to evaluate the toxicity changes. Result: The processing method of Pinelliae Rhizoma washed by hot water was as following:washed by 4 times the amount of hot water at 80 ℃ for 10 times until clear water,transfused cross-section after incision,no or slight numbness in the mouth. The average moisture,ash,extract contents of Pinelliae Rhizoma washed by hot water were 9.34%,1.71% and 4.22%,respectively. After being processed,the decline rates of weight and total acid content of Pinelliae Rhizoma were 7.49% and 43.31%. The HPLC fingerprint of Pinelliae Rhizoma before and after washing showed a decrease in all components,but there was no new chromatographic peak,and peak 9(adenosine)reduced significantly. The results of rabbit eye irritation test showed that there was no obvious eye conjunctival irritation after washing,indicating that the toxicity of Pinelliae Rhizoma decreased obviously after washing.Conclusion: The established method of Pinelliae Rhizoma by Tangxi processing is stable and feasible,the aqueous extract of Pinelliae Rhizoma has no obvious eye conjunctival irritation after washing.