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Val-9Ala and Ile + 58Thr polymorphism of MnSOD in Parkinson's disease
Authors:Vinchi Wang  Shao-Yuan Chen  Tzu-Chao Chuang  Din-E Shan  Bing-Wen Soong  Ming-Ching Kao
Institution:1. Department of Neurology, Cardinal Tien Hospital, Taipei, Taiwan;2. School of Medicine, Fu-Jen Catholic University, Taipei, Taiwan;3. Department of Hyperbaric Medicine, Cardinal Tien Hospital, Taipei;4. Department of Chemistry, Tamkang University, Tamsui, Taipei, Taiwan;5. The Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan;6. Department of Neurology, National Yang-Ming University School of Medicine, Taipei, Taiwan;7. Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan;1. Department of Chemistry, Qaemshahr branch, Islamic Azad University, Qaemshahr 47651-61964, Iran;2. House Research of Professor Reza, Education Guilan, Rasht, District 1, 41569-17139, Iran;1. Department of Physics, National Changhua University of Education, Changhua 500, Taiwan, ROC;2. Department of Physics, Tamkang University, Tamsui 251, Taiwan, ROC;1. Department of Biomedical and Environmental Analysis, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland;2. Students Scientific Society at the Department of Biomedical and Environmental Analysis, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland;1. Department of Andrology, Sexology & STDs, Faculty of Medicine, Cairo University, Cairo, Egypt;2. Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt;3. Department of Andrology, Sexology & STDs, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt;1. Department of Mathematics, Tamkang University, Tamsui, Taipei 25137, Taiwan;2. Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
Abstract:ObjectivesTo investigate the polymorphism distribution of Val-9Ala and Ile + 58Thr of the Mn-superoxide dismutase (Mn-SOD) gene among subjects with Parkinson's disease (PD) by analyses of genders and clinical severity.Design and MethodsWe examined the DNA genotypes of Val-9Ala and Ile + 58Thr from 295 PD subjects and 111 controls by nucleotide sequencing and BsaWI restriction.ResultsAla/Ala homozygosity was found in four PD subjects but not in the controls. All of the genotypes at codon + 58 among the examined samples were Ile/Ile homozygotes. Although higher carrier rate of Ala allele among PD subjects than the controls, there were no differences by analyses of the genders and clinical severity.ConclusionThe higher Ala-allele carrier rate among PD subjects may suggest a possible higher amount of mitochondrial Mn-SOD rendering higher intracellular stress in PD. In this study the polymorphisms at codons -9 and + 58 did not give informative association evidences with PD.
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