Livin gene plays a role in drug resistance of colon cancer cells |
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| Authors: | Xudong Wang Jian Xu Shaoqing Ju Hongbing Ni Jianhua Zhu Huimin Wang |
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| Affiliation: | 1. Department of Gynecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, Liaoning Province, PR China;2. Department of Medical Oncology, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, PR China;3. Department of Clinical Epidemiology and Evidence Based Medicine, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, PR China;4. Department of Medical Oncology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, Liaoning Province, PR China |
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| Abstract: | ObjectiveThe objective of this study was to investigate the effect of knockdown of Livin expression on reversing drug resistance phenotype of colon cancer HCT-8/V cells.Design and methodsSpecific short hairpin RNA (shRNA) was chosen and transfected in human colon cancer HCT-8/V cell line. Cell apoptosis and chemosensitivity were evaluated following downregulation of Livin expression.ResultsIn the current study, Livin was found to be highly expressed in the HCT-8/V colon cancer cells, which were resistant to several anti-tumor drugs. Knocking down of Livin expression in HCT-8/V cells by specific RNAi facilitated the apoptosis of HCT-8/V cells in response to vincristine (VCR), etoposide (VP-16), and 5-flourouracil (5-FU). Chemosensitivity assay confirmed the results and demonstrated the reversal of drug resistance phenotype of HCT-8/V cells.ConclusionThese data suggest that specific silencing of Livin gene expression could be a promising target for further research in clinical chemotherapy of colon cancer. |
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