Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay and by the Friedewald formula in a large community population |
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| Authors: | Kozo Tanno Tomonori Okamura Masaki Ohsawa Toshiyuki Onoda Kazuyoshi Itai Kiyomi Sakata Motoyuki Nakamura Akira Ogawa Kazuko Kawamura Akira Okayama |
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| Affiliation: | 1. Department of Hygiene and Preventive Medicine, School of Medicine, Iwate Medical University, Morioka, Japan;2. Division of Preventive Cardiology, National Cerebral and Cardiovascular Center, Osaka, Japan;3. Division of Cardiology, Department of Internal Medicine, School of Medicine, Iwate Medical University, Morioka, Japan;4. Department of Neurosurgery, School of Medicine, Iwate Medical University, Japan;5. Iwate Health Service Association, Morioka, Japan;6. The First Institute of Health Service, Japan Anti-Tuberculosis Association, Tokyo, Japan;2. The Cincinnati Eye Institute, University of Cincinnati, Cincinnati, Ohio;1. Grupo de Trabajo sobre la Dislipemia Aterogénica de la Sociedad Española de Arteriosclerosis, Spain;2. Unidad de Lípidos, Servicio de Medicina Interna, Hospital San Pedro, Logroño, La Rioja, Spain;3. Unidad de Lípidos, Servicio de Medicina Interna, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain;4. Servicio de Endocrinología, Hospital Clínico, Valencia, Spain;5. Área Sanitaria de Delicias, Atención Primaria, Zaragoza, Spain;6. Centro de Salud de Bembibre, Bembibre, León, Spain;7. Servicio de Endocrinología, Hospital Universitario Dr. Peset, Universitat de Valencia, Valencia, Spain;8. Centro de Salud de Prosperidad, Atención Primaria, Madrid, Spain;9. Unidad de Lípidos y Riesgo Vascular, Servicio de Endocrinología y Nutrición, Hospital del Mar, Universitat Autònoma de Barcelona, Barcelona, Spain;10. Unidad de Lípidos y Riesgo Vascular, Servicio de Medicina Interna, Hospital Universitario de Bellvitge, L’Hospitalet de Llobregat, Barcelona, Spain;11. Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), Centre d’Investigació Biomèdica en Xarxa-Fisiopatologia de l’Obesitat i la Nutrició (CIBEROBN), L’Hospitalet de Llobregat, Barcelona, Spain;1. Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Harbin 150081, PR China;2. Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, PE, Canada C1A 4P3;3. Department of Psychology, University of Toronto, 1265 Military Trail, Toronto, ON, Canada M1C 1A4;4. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, Canada C1A 4P3;5. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China;1. The Nutrition Doctor, Kailua-Kona, Hawaii, USA;2. Progressive Laboratories Inc., Irving, Texas, USA;2. University of Northern British Columbia. |
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| Abstract: | BackgroundWe compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population.MethodsA total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52 mmol/l were enrolled. LDL-C were directly measured by the enzymatic homogeneous assay (LDL-C (D)) and also estimated by the FF (LDL-C (F)). Paired t-test, Pearson's correlation coefficient and linear regression analysis were performed and the concordances of the National Cholesterol Education Program (NCEP) risk category were estimated.ResultsBoth in fasting (n = 3270) and nonfasting samples (n = 17,924), LDL-C (D) highly correlated with LDL-C (F): r = 0.971 and 0.955, respectively. Concordant results for NCEP categories were 84.8% for fasting samples and 80.1% for nonfasting samples. However, the bias between the 2 measurements increased in samples with TG concentrations > 1.69 mmol/l, especially in nonfasting samples.ConclusionsThe results showing less variability of the direct LDL-C assay than that of the FF in nonfasting samples suggest that epidemiological studies can use LDL-C measured by the direct assay both in fasting and nonfasting samples. |
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