Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise |
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| Authors: | Ioannis G Fatouros Athanasios Z Jamurtas Michalis G Nikolaidis Aspasia Destouni Yiannis Michailidis Christina Vrettou Ioannis I Douroudos Alexandra Avloniti Athanasios Chatzinikolaou Kiriakos Taxildaris Emmanouel Kanavakis Ioannis Papassotiriou Dimitrios Kouretas |
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| Institution: | 1. Department of Urology, University Hospital Charité, Berlin, Germany;2. Berlin Institute for Urological Research, Berlin, Germany;3. Department of Urology, Changzhou No.2 People''s Hospital, Jiangsu, China;4. Institute of Pathology, University Hospital Charité, Berlin, Germany;5. CONGEN Biotechnologie GmbH, Berlin, Germany;6. Department of Radiology, University Hospital Charité, Berlin, Germany;7. Department of Signal Transduction, Invasion and Metastasis of Epithelial Cells, Max Delbrück Center of Molecular Medicine, Berlin, Germany;1. Department of Immunology, IVI RMA, Madrid, Spain;2. Rey Juan Carlos University, Madrid, Spain;3. Health Research Institute La Fe, Valencia, Spain;4. Department of Andrology, IVI RMA, Madrid, Spain;5. Alfonso X ‘El Sabio’ University, Madrid, Spain;6. Universidad de Valencia, IVI Learning Center;7. Department of Reproductive Endocrinology and Infertility, IVI RMA, Madrid, Spain;8. Department of Reproductive Endocrinology and Infertility, IVI RMA, Roma, Italy |
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| Abstract: | ObjectivesTo determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise.MethodscfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 8 h, 10 h, 24 h).ResultscfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p < 0.001) 1 h post-exercise, remained elevated throughout recovery (52–142%, p < 0.0001), and peaked (200% rise, p < 0.0001) at 24 h post-exercise. UA and CK increased (p < 0.05), immediately post-exercise, remained elevated throughout recovery (p < 0.0001), and peaked (p < 0.0001) at 24 h of post-exercise recovery.ConclusionscfDNA sampling timing is crucial and a potential source of error following aseptic inflammation. |
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