SELDI-TOF biomarker signatures for cystic fibrosis,asthma and chronic obstructive pulmonary disease |
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| Authors: | Patrícia Gomes-Alves Margaret Imrie Robert D. Gray Paulo Nogueira Sergio Ciordia Paula Pacheco Pilar Azevedo Carlos Lopes António Bugalho de Almeida Micaela Guardiano David J. Porteous Juan P. Albar A. Christopher Boyd Deborah Penque |
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| Affiliation: | 1. Laboratório de Proteómica, Departamento de Genética, INSA-IP, Av. Padre Cruz, 1649-016 Lisboa, Portugal;2. Medical Sciences (Medical Genetics), University of Edinburgh, Molecular Medicine Centre, Western General Hospital, Edinburgh, UK;3. Departamento de Epidemiologia, INSA-IP, Lisboa, Portugal;4. Laboratory of Proteomics, CNB-CSIC, Universidad Autónoma de Madrid, Madrid, Spain;5. Unidade de Biologia Molecular, Departamento de Genética, INSA-IP, Lisboa, Portugal;6. Clínica Universitária de Pneumologia, Hospital Santa Maria, Lisboa, Portugal;7. Hospital São João, Universidade do Porto, Porto, Portugal;2. Biology consultant, Max-Baermann-Str. 21, 51429 Bergisch Gladbach, Germany;1. EPIUnit – Institute of Public Health, University of Porto, Portugal;2. Department of Mathematics, Faculty of Sciences, University of Porto, Portugal;3. Centre of Mathematics of the University of Porto, Portugal;4. Faculty of Medicine of the University of Porto, Portugal;5. Pulmonology Department, Centro Hospitalar de Vila Nova de Gaia/Espinho, EPE, Vila Nova de Gaia, Portugal;6. National Centre for Multidrug-resistant Tuberculosis, Portugal;1. Makerere University College of Health Sciences, Kampala, Uganda;2. Joint Clinical Research Centre, Kampala, Uganda;3. Blizard Institute, Queen Mary University of London, UK;4. Zvitambo Institute for Maternal and Child Health Research, Harare, Zimbabwe;5. Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA;1. Departamento de Neumología, Centro Hospitalar de São João-EPE, Porto, Portugal;2. Departamento de Neumología, Centro Hospitalar de Lisboa Norte-EPE/Hospital Pulido Valente, Lisboa, Portugal;3. Departamento de Medicina Interna, Centro Hospitalar de Lisboa Central-EPE/Hospital Curry Cabral, Lisboa, Portugal;4. Departamento de Anatomía Patológica, Centro Hospitalar de Lisboa Central-EPE/Hospital Curry Cabral, Lisboa, Portugal;5. Ribeiro Sanches Pulmonary Diagnostic Center, Lisboa, Portugal |
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| Abstract: | Objectives:The aim of this work was to establish protein profiles in serum and nasal epithelial cells of cystic fibrosis individuals in comparison with controls, asthma and chronic obstructive pulmonary disease patients for specific biomarker signatures identification.Design and methods:Protein extracts were analyzed by Surface Enhanced Laser Desorption/Ionization Time-Of-Flight Mass-Spectrometry (SELDI-TOF-MS).Results:The mass spectra revealed a set of peaks with differential expression in serum and nasal cells among the different groups studied, resulting into peak signatures representative/specific of each pathology. Logistic regressions were applied to those peaks; sensitivity, specificity, Youden's indexes and area under the curve (AUC) of the respective receiver operating characteristic (ROC) curves were compared.Discussion:Multivariate analysis demonstrated that combination of peaks has a better predictive value than the individual ones. These protein signatures may serve as diagnostic/prognostic markers for the studied diseases with common clinical features, or as follow-up assessment markers of therapeutic interventions. |
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