不同丛枝菌根真菌组合对滇重楼光合生理和化学成分的影响

目的:研究接种不同丛枝菌根(AM)真菌组合对滇重楼幼苗的生长发育和入药品质的影响,为培育优质滇重楼药材提供参考.方法:通过室温盆栽接种试验方法,在灭菌栽培土壤中分别接种9组不同AM真菌组合,以不接种为空白组,观察不同AM真菌组合对滇重楼幼苗的根系侵染率、光合参数、生理指标及化学成分等的影响.结果:接种外源AM真菌组合显...

Effect of Different Combinations of Arbuscular Mycorrhiza on Paris polyphylla var. yunnanensis and Chemical Components

Objective:To study the effects of different arbuscular mycorrhizal(AM)fungi combinations on the growth and quality of Paris polyphylla var. yunnanensis seedlings,in order to provide reference for the cultivation of high quality P. polyphylla var. yunnanensis. Method: Through the room temperature pot inoculation test method,nine groups of different AM fungi combinations were inoculated into sterilized soil,and the control group was not inoculated. The effects of different AM fungi combinations on root infection rate,photosynthetic parameters,physiological indexes and chemical components of P. polyphylla var. yunnanensis seedlings were observed. Result: The spore density in rhizosphere soil,the infection rate and intensity of AM fungi in roots,and the activities of succinate dehydrogenase and alkaline phosphatase in roots of P. polyphylla var. yunnanensis were significantly increased by inoculating the combination of exogenous AM fungi. Compared with the control group,the contents of chlorophyll,malondialdehyde,soluble sugar,soluble protein content and the activities of antioxidant enzymes in leaves of P. polyphylla var. yunnanensis were increased in different AM fungi treatment groups. The content of total saponin increased from T1 to T2,decreased rapidly from T2 to T4,and increased at T5. Conclusion: Different mixed AM fungus can form a good symbiotic relationship with the roots of P. polyphylla var. yunnanensis,improve the mycorrhizal vitality,enhance the resistance of P. polyphylla var. yunnanensis to adverse environment,promote the growth and development of P. polyphylla var. yunnanensis and improve the quality of rhizome. According to the comprehensive inoculation effect,the combination of S5,S8 and S9 AM fungus has the best effect,which provides reference value for the application of mycorrhizal biotechnology of P. polyphylla var. yunnanensis.