基于网络药理学研究甘草-石榴皮复配抗紫外线致皮肤光损伤的保护作用机制

目的:应用网络药理学的方法及工具研究甘草-石榴皮复配的潜在协同保护皮肤光损伤作用机制,为中药复方现代化及新药创新发现提供依据.方法:利用中药系统药理学数据库与分析平台(TCMSP)数据库结合所查文献,获得甘草-石榴皮复配后的活性成分及所对应的靶标;将所得靶标输入蛋白质数据库(UniProt)中,查询靶标对应的基因名称....

Protective Effect of Glycyrrhizae Radix et Rhizoma-Granati Pericarpium Formula on Ultraviolet-induced Skin Damage

Objective:To explore the potential synergistic protective mechanism of Glycyrrhizae Radix et Rhizoma-Granati Pericarpium formula compound by using the methods and tools of network pharmacology,and provide a basis for the modernization of traditional Chinese medicine(TCM)compounds and the discovery of new drugs. Method: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)was used to obtain the active components of Glycyrrhizae Radix et Rhizoma-Granati Pericarpium formula and their corresponding targets. The obtained targets were input to the UniProt database to inquire the gene names corresponding to the targets. By searching the CTD database,Genecards database and OMIM database of disease-related websites,the anti-sunburn targets were obtained. The interaction of the active targets was analyzed with online STRING database to screen the predicted core targets. The gene ontolog(GO)gene function enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of the predictive targets were performed by using DAVID database. Cytoscape 3.6.1 software was used to make drug-component-target network diagram, protein-protein interaction network diagram and component-target-pathway network diagram. Online website Draw Venn Diagram was used to show the relationship between disease targets and drug predicted targets. R Studio software was used to draw the functional enrichment analysis diagram of GO gene and KEGG pathway. Molecular docking between the active ingredients and the core targets was performed using GOLD software. Result: The 16 active compounds were collected,such as liquiritin,glycyrrhizin,kaempferol and quercetin. The active components mainly acted on 5 core targets:protein kinase B1(AKT1),interleukin(IL)-6,vascular endothelial growth factor(VEGFA),tumor necrosis factor(TNF)and tumor suppressor gene(TP53)and played a role in anti-sunburn effect primarily through these pathways such as hepatitis B,pathways in cancer,toxoplasmosis,chagas disease(American trypanosomiasis),and TNF signaling pathway. Conclusion: Based on the method of network pharmacology,the present study has preliminarily explored the anti-sunburn targets and pathways of Glycyrrhizae Radix et Rhizoma-Granati Pericarpium formula,and further verified the characteristics of multi-component and multi-target treatment of diseases in TCM,so as to provide certain scientific ideas for the modernization research of Chinese herbal compound prescriptions.