糖尿病胃轻瘫动物模型建立方法初探

目的探讨糖尿病胃轻瘫(diabetic gastroparesis,DGP)大鼠的造模方法。方法从70只正常SD雄性大鼠中随机选择60只高脂高糖饲养2周后,禁食、自由饮水12 h,腹腔注射溶于柠檬酸盐缓冲液的链脲佐菌素(streptozotocin,STZ)50 mg/kg建立糖尿病大鼠模型。从50只糖尿病大鼠中随机选取10只高脂高糖饮食喂养8周,设为DGP模型组。余10只健康SD大鼠普通饲料饲养2周后腹腔注射等剂量柠檬酸盐缓冲液普通饲料喂养8周,设为对照组。检测造模期间大鼠血糖、体重变化及胃肠动力指标。结果与正常组相比,模型组造模后空腹血糖显著增加(P<0.01),8周后体重明显下降(P<0.01),胃残留率增加(P<0.05),小肠推进率显著降低(P<0.01)。结论在腹腔注射STZ配合高脂高糖饲养诱导SD大鼠形成糖尿病基础上,高脂高糖喂养可构建DGP大鼠模型。

Establishment of An Animal Model of Diabetic Gastroparesis

Objective To explore a method of establishing a rat model for diabetic gastroparesis(DGP).Methods Sixty rats were randomly chosen from seventy healthy SD male rats as a model group that was fed with a highfat and high-sugar diet for two weeks.After being fasted and freely drinking water for 12h,these rats were injected intraperitoneally with streptozotocin(STZ soluble in 0.1mol/L citric acid buffered salt solution)50mg/kg to establish a diabetic rat model.Fasting blood glucose≥13.9mmol/L after 72h indicated that a proper diabetic animal model was induced.From the fifty SD rats in the diabetic model,ten were randomly chosen to feed on a high-fat and high-sugar diet for eight weeks to establish a DGP animal model.Ten healthy SD male rats in the control group were fed for two weeks.After being fasted and freely drinking water for 12 h,they were injected intraperitoneally with an equivalent dosage of sodium citrate buffer and then fed with an ordinary diet for eight weeks as the normal control group.During the experiment,the general condition,body weight and blood glucose of rats in both groups were observed,while the gastric residual rate and small intestine advancing rate were measured after the rats were sacrificed.Results Compared with the normal group,the fasting blood glucose of the model group was significantly increased after modeling(P<0.01),and the rats became thinner,whose body weight and small bowel propulsion rate(P<0.01)were significantly reduced,while the gastric residue rate was significantly increased(P<0.01,P<0.05)after eight weeks.Conclusion Eight weeks of intraperitoneal injection of STZ 50mg/kg combined with a high-fat and high-sugar diet can induce a DGP rat model.