甲基化酶抑制剂对变应性鼻炎大鼠Th1/Th2细胞及相关因子的影响

目的:探讨甲基化酶抑制剂5-杂氮-2-脱氧胞苷(5-aza-2-deoxycytidine，5-aza)对变异性鼻炎大鼠辅助性T细胞(helper T cell，Th)亚群Th1和Th2细胞的分化及白细胞介素(interleukin，IL)-4、干扰素(interferon，IFN)-γ水平表达的影响。方法:将30只S...

Effects of methylase inhibitors on Th1/Th2 cells and related factors in allergic rhinitis rats

Objective To investigate the effects of 5-aza-2-deoxycytidine(5-aza)on the differentiation of helper T cell(Th1)and Th2 cells and the expression of interleukin(IL)-4 and interferon(IFN)-γin rats with variant rhinitis.Methods Thirty SD male rats were randomly divided into 3 groups:normal group,model group and 5-aza group with 10 rats in each group.Allergic rhinitis model was established by ovalbumin sensitization.After intervention,blood samples were collected for flow cytometry to detect the ratio of Th1 and Th2 cells,and enzyme linked immunosorbent assay(ELISA)was used to detect the expression of cytokine IFN-γsecreted by Th1 cells and IL-4 secreted by Th2 cells.The nasal mucosa was stained with hematoxylin eosin staining(HE).Results The results of flow cytometry showed that the ratio of Th2 cells in model group was significantly higher than that in 5-aza group and normal group[(7.28±1.37)%vs(6.17±1.06)%vs(6.05±0.95)%,F=2.920,P<0.05].The ratio of Th2 in the 5-aza group was significantly lower than that in the model group(P=0.039).There was no significant difference in the Th1 ratio among the three groups(P>0.05).The results of ELISA showed that the expression level of IL-4 in the model group was significantly higher than that in the normal group and 5-aza group[(175.19±10.14)pg/mL vs(160.14±16.86)pg/mL,(160.75±17.45)pg/mL,F=2.743,P<0.05].The expression level of IL-4 in 5-aza group was significantly lower than that in model group(P=0.047).There was no significant difference in IFN-γexpression among the three groups(P>0.05).The light microscope of HE staining showed that the structure of nasal mucosa was complete and clear,without edema and hyperemia in the normal group.In the model group,hyperemia and edema of lamina propria,hyperplasia of glands and blood vessels in submucosa,infiltration of inflammatory cells and eosinophils,and destruction of nasal mucosa cilia were observed.In the 5-aza group,the nasal mucosa was almost intact with a small amount of eosinophils.Conclusion Methylase inhibitors can effectively alleviate the symptoms of allergic rhinitis and reduce the nasal mucosa injury of allergic rats.It is suggested that methylase inhibitors may down regulate Th2 expression and inhibit inflammatory reaction.