| 小鼠颌下腺神经生长因子的提取及活性鉴定的初步研究 |
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| 引用本文： | 熊仁平 周元国. 小鼠颌下腺神经生长因子的提取及活性鉴定的初步研究[J]. 中国生化药物杂志, 1996, 17(3): 111-113 |
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| 作者姓名： | 熊仁平 周元国 |
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| 作者单位： | 第三军医大学野战外科研究所四室!重庆630042 |
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| 摘 要： | 对神经生长因子（Nerve Growth Factor,NGF）提取纯化、活性鉴定进行了研究。将小鼠颌下腺组织匀浆、离心,取其上清,在不同pH和离子强度,依次通过两个离子交换纤维素层析柱,制得 2.5 S NGF纯品。其分子量约43.5 kD,3种主要组分的等电点分别为8.85、9.10和9.20。用鸡胚背根培养法证明其具有刺激神经生长的作用。
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| 关 键 词： | 神经生长因子 提取 生物活性 |
Isolation of the Nerve Growth Factor from Male Rat Maxillary Glands |
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| Xiong Renping,Zhou Yuanguo,Zhu Peifang,Zhou Jihong,Liu DaweiResearch Institute of Surgery,Third Military Medical College of PLA. Isolation of the Nerve Growth Factor from Male Rat Maxillary Glands[J]. Chinese Journal of Biochemical Pharmaceutics, 1996, 17(3): 111-113 |
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| Authors: | Xiong Renping Zhou Yuanguo Zhu Peifang Zhou Jihong Liu DaweiResearch Institute of Surgery Third Military Medical College of PLA |
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| Affiliation: | Chongqing 630042 |
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| Abstract: | The nerve growth factor (NGF) was isolated from male rat maxillary glands. After homog-enization and centrif ugation of the glands, the suqernatant was sequentially chromatogaphed at various pH, on CM cellulose columns. NGF was isolated in this fashion. The molecular weight of the purfied NGF is about 13. 5 kD. The isoelectric points of three fractions of the purified product are 8. 85, 9. 10 and 9. 20 respectively. Using chicken embryo dorsal root ganglia assay, the biological activity of NGF was determined. |
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| Keywords: | Nerve growth factor Isolation Bioassay of activity |
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