| ~(59)Fe摄入法和ELISA法测定重组人红细胞生成素活性的比较 |
| |
| 引用本文： | 刁勇 古卓良. ~(59)Fe摄入法和ELISA法测定重组人红细胞生成素活性的比较[J]. 中国生化药物杂志, 1996, 17(6): 231-233 |
| |
| 作者姓名： | 刁勇 古卓良 |
| |
| 作者单位： | 南京军区药品检验所!南京210002(刁勇，古卓良)，中国药品生物制品检定所(周勇，程亚琴)，南京军区医学研究所(聂海洋，吴刚) |
| |
| 摘 要： | 对重组人红细胞生成素（rhEPO）活性测定的两种方法进行了比较。认为标准方法（~(59)Fe摄入法）结果准确,但操作复杂。而ELISA法必须与其它纯度检查法如SDS-PAGE、IEF、HPLC等相结合才能得出准确结果。对于高纯度结构完整的rhEPO分子,两种方法结果应一致。
|
| 关 键 词： | 重组人红细胞生成素 活性测定 |
Comparison of rhEPO Activity between 59Fe Incorporation (Polycythaemie Mouse) and ELISA Method |
| |
| Diao Yong,Gu Zuoliang,Zhou Yong,Cheng Yaqin,Nie Haiyang,Wu Gang Nanjing Military Institute fr Drug Contral. Comparison of rhEPO Activity between 59Fe Incorporation (Polycythaemie Mouse) and ELISA Method[J]. Chinese Journal of Biochemical Pharmaceutics, 1996, 17(6): 231-233 |
| |
| Authors: | Diao Yong Gu Zuoliang Zhou Yong Cheng Yaqin Nie Haiyang Wu Gang Nanjing Military Institute fr Drug Contral |
| |
| Affiliation: | Nanjing 210002 |
| |
| Abstract: | The in vivo and in vitro activity assay methods of rhEPO were compared. As the standard method, 59Fe Incorporation Method using the polycythaernic mouse was accuracy, but it's timeconsum-ing and complicated. The EL1SA method is quicker and more sensitive, but is must combine with other techniques such as SDS-PAGE,IEF and HPLC etc,to obtain accurate results. To intact EPO molecular of high purity, the results of the two methods should accord with each other. |
| |
| Keywords: | rhEPO Activity determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
