| 高效液相色谱法测定尿中氨苄青霉素的浓度 |
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| 引用本文： | 赵春杰,何春馥,赵慧茹,解健博,吴起娟. 高效液相色谱法测定尿中氨苄青霉素的浓度[J]. 沈阳药科大学学报, 1990, 0(1) |
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| 作者姓名： | 赵春杰 何春馥 赵慧茹 解健博 吴起娟 |
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| 作者单位： | 沈阳药学院药物分析教研室(赵春杰，何春馥，赵慧茹，解健博)，沈阳药学院药物分析教研室(吴起娟) |
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| 摘 要： | 本文报道反相高效液相色谱法测定尿中氨苄青霉素的浓度。该法采用国产高效液相色谱仪(YSB-2型),YWG-C_(18)10μm柱(150×5mm),磷酸盐缓冲液-甲醇-乙腈(82:8:10)为流动相,紫外检测器测定波长254mm。尿中氨苄青霉素在2～10μg的范围内呈线性关系。低浓度和高浓度的回收率分别是99.7±1.68%和100.7±1.05%。三名受试者尿排泄率分别为27.2%,20.7%和21.0%。
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| 关 键 词： | 高效液相色谱法 氨苄青霉素 |
HPLC-DETERMINATION OF AMPICILLIN IN URINE |
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| Zhao Chunjie,He Chunfu,Zhao Huiru,Xie Jianbo,Wu Qijuan. HPLC-DETERMINATION OF AMPICILLIN IN URINE[J]. Journal of Shenyang Pharmaceutical University, 1990, 0(1) |
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| Authors: | Zhao Chunjie He Chunfu Zhao Huiru Xie Jianbo Wu Qijuan |
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| Affiliation: | Zhao Chunjie,He Chunfu,Zhao Huiru,Xie Jianbo,Wu Qijuan,Department of Pharmaceutical Analysis |
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| Abstract: | A Rp-HPLC method has been developed for the quantitative determination ofampicillin in urine. The chromatographic conditions were:a YWC-C_(-18)(10μm) reve-rsed-phase column(150×50mm), an acid phosphate-methanol-acetonitrile(82:8:10)mobile phase; UV detector at 254nm. The mean recovery of ampicillin at low andhigh concentration were. 99.7+1.68% and 100.74±1.05% respectively. Goodlinear range was 2μg~10μg. The ratios of the excreted portion to the administereddose(500mg/capsule) in three tests were 27.2%. 20.7% and 21.0% respectively. |
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| Keywords: | HPLC Ampicillin |
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