| 不对称水溶性菁染料的简便制备与纯化方法 |
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| 引用本文： | 楼开炎,钱旭红,宋恭华. 不对称水溶性菁染料的简便制备与纯化方法[J]. 医学教育探索, 2002, 0(2): 212-215 |
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| 作者姓名： | 楼开炎 钱旭红 宋恭华 |
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| 作者单位： | 华东理工大学药物化工研究所 上海200237(楼开炎，钱旭红)，华东理工大学药物化工研究所 上海200237(宋恭华) |
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| 摘 要： | 以对肼基苯磺酸为起始原料，经6步反应合成了2个可用于DNA荧光标记的不对称水溶性菁染料，采用三元展开剂体系，用制备薄层色谱方法分离提纯各中间体和产物，得到了与文献报道制备液相色谱方法相当的收率。
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| 关 键 词： | 水溶性菁染料 合成 纯化 制备薄层色谱 |
Synthesis and Purification of Unsymmetrical Water-soluble Cyanine Dyes |
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| LOU Kai yan,QIAN Xu hong,SONG Gong hua. Synthesis and Purification of Unsymmetrical Water-soluble Cyanine Dyes[J]. Researches in Medical Education, 2002, 0(2): 212-215 |
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| Authors: | LOU Kai yan QIAN Xu hong SONG Gong hua |
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| Abstract: | Two water soluble unsymmetrical cyanine dyes, which can be used as fluorescence tag in DNA labeling, was synthesized from p hydrazino benzenesulfonic acid by 6 steps. A ternary solvent were developed and used as eluant in purification of intermediates and final dyes by PTLC method. The yields were as good as that of the HPLC method that had been reported. |
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| Keywords: | water soluble cyanine synthesis purification PTLC |
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