中药复方藿莲香Ⅰ号的遗传毒理学研究 |
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引用本文: | 吴彦,;杨鸿,;钟菊迎,;刘钊,;高尔宁,;王萍娟,;黄东业,;田兆福,;白家峰.中药复方藿莲香Ⅰ号的遗传毒理学研究[J].中国药物警戒,2014(11):649-651. |
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作者姓名: | 吴彦 ;杨鸿 ;钟菊迎 ;刘钊 ;高尔宁 ;王萍娟 ;黄东业 ;田兆福 ;白家峰 |
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作者单位: | [1]广西中烟工业有限责任公司技术中心,广西南宁530001; [2]中国中医科学院医学实验中心,北京100700 |
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基金项目: | 桂工信科技[2014]529. |
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摘 要: | 目的:根据前期藿莲香Ⅰ号药效学研究结果,进一步研究该复方的急性毒性和致突变作用,为其进一步应用的安全性提供理论依据。方法应用急性毒性实验、小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验检测该组分配伍复方的急性毒性和致突变性。结果急性经口毒性试验:雌性、雄性小鼠经口MTD均大于10g·kg-1,属实际无毒。致突变实验:小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验结果均为阴性,显示在本实验条件下,该中药复方未见有致突变性作用。结论中药复方藿莲香Ⅰ号未见有明显的急性毒性和致突变作用,表明该药物安全性良好。
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关 键 词: | 藿莲香Ⅰ号 急性毒性 微核 染色体畸变试验 Ames试验 |
Research on the Acute and Mutagenic Toxic Effect of Huolianxiang Compound |
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Institution: | WU Yan, YANG Hong, ZHONG Ju-ying, LIU Zhao, GAO Er-ning, WANG Ping-juan, HUANG Dong-ye, TIAN Zhao-fu, BAI Jia-feng (1Experimental Research Center, China Academy of Chinese Medical Science. Beijing 100700, China; 2Technical Center, China Tobacco Guangxi Industrial COLTD, Guangxi Nanning 530001, China) |
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Abstract: | Objective To further evaluate acute toxicity and mutagenicity of Huolianxiang-I on the basis of previous pharmacodynamics results in order to provide theory basis for its further application. Methods The acute toxicity and mutagenecity of Huolianxiang-I were examined by acute oral toxicity test, mouse bone marrow polychromatic erythrocyte micronucleus test,mouse bone marrow chromosome aberration test and Ames test. Results For acute oral toxicity, maxi-mum tolerance doses of male and female mice are higher than 10g·kg-1 which are actually innocuity.For mutagenicity, negative results were seen in mouse bone marrow polychromatic erythrocyte micronucleus test,mouse bone marrow chromose aberration test and Ames test showing that Huolianxiang-I has no mutagenecity in this study. Conclusion No evident acute toxicity and mutagenicity are found in this study demonstrating that Chinese medicine compound Huo-lianxiang-I is safe. |
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Keywords: | Huolianxiang compound acute toxicity micronucleus test chromosomal aberration test Ames test |
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