| 三七总皂苷对烫伤大鼠心肌Gsα-mRNA表达的影响 |
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| 引用本文： | 张海港,李晓辉,李淑惠,徐红梅,谭娅. 三七总皂苷对烫伤大鼠心肌Gsα-mRNA表达的影响[J]. 中草药, 2001, 32(11): 1000-1002 |
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| 作者姓名： | 张海港 李晓辉 李淑惠 徐红梅 谭娅 |
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| 作者单位： | 第三军医大学药理教研室, |
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| 基金项目： | 国家自然科学基金资助项目(No.39370791) |
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| 摘 要： | 目的研究三七总皂苷(panax notoginseng saponins,PNS)对烫伤早期大鼠心肌Gsα mRNA表达的影响.方法通过30%体表面积皮肤Ⅲ度烫伤大鼠模型,应用dot blotting杂交、心肌组织原位杂交、放射免疫分析等技术观察三七总皂苷对严重体表烫伤大鼠心肌Gsα mRNA表达水平、cAMP产生量、腺苷酸环化酶(adenylylcyclase,AC)活性的影响.结果大鼠心肌组织中Gsα mRNA表达量、cAMP含量、AC活性在烫伤后3 h均明显降低.100,200 mg/kg PNS明显增加烫伤大鼠心肌组织Gsα mRNA的表达,增加量与PNS剂量呈显著正相关(r=0.95,P＜0.05).100,200 mg/kg使烫伤大鼠心肌组织cAMP含量分别增加21%,36%,AC活性分别增强40%,63%(P＜0.01).结论 PNS可增加烫伤大鼠心肌GsαmRNA表达,增强AC活性,增加cAMP生成.
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| 关 键 词： | 烧伤 兴奋性G蛋白 大鼠 信号转导 Gsα mRNA 三七总皂苷 腺苷酸环化酶 环－磷酸腺苷 |
| 文章编号： | 0253-2670(2001)11-1000-03 |
| 收稿时间： | 2000-02-10 |
| 修稿时间： | 2000-02-10 |
Effect of Panax notoginseng saponins on myocardial Gsα-mRNA expression of seriously scalded rat |
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| ZHANG Haigang,LI Xiaohui,LI Shuhui,XU Hongmei and TAN Ya. Effect of Panax notoginseng saponins on myocardial Gsα-mRNA expression of seriously scalded rat[J]. Chinese Traditional and Herbal Drugs, 2001, 32(11): 1000-1002 |
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| Authors: | ZHANG Haigang LI Xiaohui LI Shuhui XU Hongmei TAN Ya |
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| Affiliation: | Department of Pharmacology, Third Military Medical University, Chongqing 400038, China;Department of Pharmacology, Third Military Medical University, Chongqing 400038, China;Department of Pharmacology, Third Military Medical University, Chongqing 400038, China;Department of Pharmacology, Third Military Medical University, Chongqing 400038, China;Department of Pharmacology, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | |
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| Keywords: | burn stimulatory G protein message RNA signal transduction Panax notoginseng saponins (PNS) adenyl cyclase cyclic AMP |
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