肺癌组织中乙酰肝素酶与血管内皮生长因子-C的表达

目的　探讨乙酰肝素酶 (HPSE)和血管内皮生长因子 C(VEGF C)的表达与肺癌发生、发展的关系。方法　应用免疫组织化学SABC法对 6 5例肺癌 (其中鳞癌 31例、腺癌 2 5例、大细胞癌3例、小细胞癌 6例 )患者的肺癌组织中HPSE和VEGF C蛋白的表达进行测定 ,并与癌旁组织和正常肺组织对比 ,结合肺癌的临床病理学特征及预后进行分析。结果　肺癌组织中HPSE和VEGF C蛋白的阳性率分别为 5 1% ( 33/6 5 )和 5 7% ( 37/6 5 ) ,明显高于正常肺组织的 5 % ( 3/6 5 )、6 % ( 4 /6 5 )和癌旁组织 9% ( 6 /6 5 )和 12 % ( 8/6 5 ) ( χ2 =34 6 ,38 8和 2 6 7,2 8 6 ;均P <0 0 1) ;不同病理类型、分化程度的肺癌组织中HPSE和VEGF C蛋白阳性率差异均无显著性 ( χ2 =0 39和 0 4 1,0 4 5和 0 0 4 ,均P >0 0 5 ) ;Ⅲ、Ⅳ期患者肺癌组织中HPSE和VEGF C蛋白阳性率均为 95 % ( 2 1/2 2 ) ,明显高于Ⅰ、Ⅱ期患者的 2 8% ( 12 /43)和 37% ( 16 /43) ( χ2 =2 6 6和 2 0 1,P <0 0 1) ;生存 3年或以下的肺癌组织中HPSE和VEGF C蛋白阳性率 88% ( 2 2 /2 5 )和 96 % ( 2 4 /2 5 )明显高于生存 3年或以上者的 2 8%( 10 /36 )和 36 % ( 13/36 ) ( χ2 =2 1 5和 2 2 2 ,P <0 0 1)。结论　肺癌组织中存在HPSE和VEGF C的高表达 ,

The expression of heparanase and vascular endothelial growth factor-C in human lung cancer

OBJECTIVE: To investigate relationship between the expression of heparanase (HPSE) and vascular endothelial growth factor-C (VEGF-C) and tumorigenesis, progression in human lung cancer. METHODS: The expression of HPSE and VEGF-C protein in 65 cases of lung cancer, adjacent tissues of cancer and normal tissues was tested by immunohistochemical SABC method and analysed by clinico-pathological characteristics and prognosis of lung cancer. RESULTS: The rate of expression of HPSE and VEGF-C protein in tumor tissues (51% and 57%) was significantly higher than that in adjacent tissues of cancer (9% and 12%) and normal tissues (5% and 6%) (chi2 = 34.6, 38.8, 26.7, 28.6; P < 0.01); It was shown that HPSE and VEGF-C protein expression did significantly not correlate with the type (chi2 = 0.39, 0.41, P > 0.05) and grade of the tumor (chi2 = 0.45, 0.04, P > 0.05); but it correlated with the clinical stage (chi2 = 26.6, 20.1; P < 0.01) and survival time of the patients (chi2 = 21.5, 22.2; P < 0.01). CONCLUSIONS: The results suggest that there be overexpression of HPSE and VEGF-C protein in lung cancer tissues, and which perhaps participate in regulation of tumorigenesis, progression in lung cancer. The expressions of HPSE and VEGF-C protein are used as an useful marker of the biological behavior of lung cancer and as an independent prognosis factor for the patients with lung cancer.