| TH基因修饰的胶质细胞脑内移植治疗帕金森病的实验研究 |
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| 引用本文： | 刘俊,张可成,蔡文琴. TH基因修饰的胶质细胞脑内移植治疗帕金森病的实验研究[J]. 第三军医大学学报, 2001, 23(5): 556-558 |
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| 作者姓名： | 刘俊 张可成 蔡文琴 |
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| 作者单位： | 1. 第三军医大学附属新桥医院神经外科,
2. 第三军医大学基础医学部组织学与胚胎学教研室, |
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| 基金项目： | 国家自然科学基金资助项目! ( 39370 686) |
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| 摘 要： | 目的 通过ex vivo途径，利用基因工程化细胞对酪氨酸羟化酶（TH）基因在治疗帕金森病中的作用进行探讨。方法 将TH基因构建人逆转录病毒载体中，再将含重组DNA的病毒上清感染培养的星形胶质细胞移植入PD大鼠脑内，了解PD鼠旋转行为的改善情况。结果 TH基因治疗组PD鼠旋转行为改善明显，结论 TH对PD有明显的治疗作用。星形胶质细胞可作为有效的基因工程细胞。
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| 关 键 词： | 帕金森病 基因治疗 TH基因 胶质细胞移植 重组DNA |
| 文章编号： | 1000-5404(2001)05-0556-03 |
| 修稿时间： | 2000-07-10 |
Experimental study of intracerebral transplantation of TH gene modified astrocytes for treating Parkinson's disease |
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| LIU Jun,ZHANG Ke-cheng,CAI Wen-qin. Experimental study of intracerebral transplantation of TH gene modified astrocytes for treating Parkinson's disease[J]. Acta Academiae Medicinae Militaris Tertiae, 2001, 23(5): 556-558 |
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| Authors: | LIU Jun ZHANG Ke-cheng CAI Wen-qin |
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| Abstract: | Objective To explore the role of tyrosine hydroxylase (TH) gene in the treatment of Parkinson's disease (PD) by using ex vivo gene transfer. Methods After the construction of TH gene in a retroviral vector, the astrocytes were cultured with the supernatant containing the recombinant DNA and then grafted into the cerebrum of PD rats. The reduction of the rat rotation beharior was evaluated. Results The rotation of PD rats was markedly improved in the rats with ex vivo gene transfer. Conclusion TH has an obvious efficiency on the treatment of PD and the astrocytes can be used as effective gene transfer cells. |
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| Keywords: | Pakinson's disease gene therapy TH gene |
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