The influence of age,race, and gender on peripheral blood mononuclear-cell subsets in healthy nonsmokers |
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Authors: | D. J. Tollerud J. W. Clark L. Morris Brown C. Y. Neuland L. K. Pankiw-Trost W. A. Blattner R. N. Hoover |
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Affiliation: | (1) Environmental Epidemiology Branch, National Cancer Institute, Bethesda, Maryland;(2) Present address: Channing Laboratory, 180 Longwood Avenue, 02115 Boston, Massachusetts;(3) Present address: Braton Biotech, Rockville, Maryland;(4) National Cancer Institute, Executive Plaza North, Room 434, 20892 Rockville, Maryland |
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Abstract: | To investigate the influence of age, race, and gender on the cellular immune system, we determined T-cell, B-cell, monocyte, natural killer (NK)-cell, and HLA-DR+-cell subsets in 266 nonsmokers from a population-based random sample of healthy adults using monoclonal antibodies and flow cytometry. Blacks had a lower total white blood-cell count than whites (P 0.0001), due primarily to a decrease in granulocytes. There was no significant difference in absolute lymphocyte count between blacks and whites. Blacks had a higher proportion of CD19+ cells (Leu 12+ B cells) and a lower proportion of CD3+ cells (OKT3+ T cells) than whites (P 0.01). Female sex and increasing age were independently associated with an increased percentage of CD4+ cells (OKT4A+ helper-inducer T-cell subset), resulting in a higher helper/suppressor ratio among women and older individuals (P 0.05). Black race and increasing age were independently associated with an increased proportion of HLA-DR+ cells (P 0.0001) which was not attributable to B cells or monocytes. No significant age, race, or gender effects were observed for CD14+ cells (Leu M3+ monocytes) or CD16+ cells (Leu 11A+ natural killer cells). These data demonstrate that age, race, and gender are each associated with significant differences in peripheral blood monouclear-cell subsets. Population-based data such as these provide an important foundation for future design and interpretation of human flow cytometry data. |
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Keywords: | Lymphocyte subsets normal values flow cytometry age race gender |
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