| 钙通道开放和肾上腺素受体兴奋对La3+跨豚鼠心肌细胞膜行为的影响 |
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| 引用本文： | 陈榕,席小莉,杨频. 钙通道开放和肾上腺素受体兴奋对La3+跨豚鼠心肌细胞膜行为的影响[J]. 海南医学院学报, 2011, 17(7): 879-881,885. DOI: CNKI:46-1049/R.20110613.1457.006 |
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| 作者姓名： | 陈榕 席小莉 杨频 |
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| 作者单位： | 1. 海南医学院药学院,海南海口,571101
2. 山西大学分子科学研究所,山西太原,030006 |
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| 基金项目： | 太原市科技局知识产权局资助项目 |
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| 摘 要： | 目的:研究钙通道开放和肾上腺素受体兴奋对稀土La3+跨豚鼠心肌细胞膜行为的影响.方法:使用Fura-2荧光探针方法,测量340 nm/380 nm的荧光强度比的变化,检测La3+的跨膜行为.结果:豚鼠心肌细胞在细胞外La3+浓度为0.01～0.1 mmol/L时,使用钙通道激动剂Bay K8644、高钾KCl(35 m...
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| 关 键 词： | 豚鼠 心肌细胞 La3+ 跨膜 荧光探针 钙通道 肾上腺素受体 |
Effect of open calcium channel and adrenoceptor stimulation on La3+ transmembrane behavior of guinea pig ventricular myocytes |
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| CHEN Rong,XI Xiao-li,YANG Pin. Effect of open calcium channel and adrenoceptor stimulation on La3+ transmembrane behavior of guinea pig ventricular myocytes[J]. Journal of Hainan Medical College, 2011, 17(7): 879-881,885. DOI: CNKI:46-1049/R.20110613.1457.006 |
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| Authors: | CHEN Rong XI Xiao-li YANG Pin |
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| Affiliation: | 1.Department of Pharmacy,Hainan Medical University,Haikou 571101,China;2.Institute of Molecular Science,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | Objective： To study the effect of open calcium channel and adrenoceptor stimulation on La3＋ transmembrane behavior of guinea pig ventricular myocytes.Methods： The change of 340/380nm fluorescence intensity ratio was detected by Fura-2 fluorescence probe technique to determine La3＋ transmembrane behavior.Results： Although the voltage-dependent calcium channel（VDCC） was opened by calcium channel agonist Bay K8644 and KCl（35mmol/L） depolarization,La3＋ could not enter into guinea pig ventricular myocytes under extracellular La3＋ concentration of0.01-0.1mmol/L.Stimulation of adrenoceptor by adrenaline and isoprenaline can induce Ca2＋ influx,but La3＋ still can not enter guinea pig ventricular myocytes.Conclusions： La3＋ transmembrane is not promoted by opening of VDCC and stimulating adrenoceptor. |
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| Keywords: | Guinea pig； Ventricular cells； La3＋； Transmembrane； Fluorescence probe； Calcium channel； Adrenoceptor； |
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