嗅球注射甲苯对小鼠嗅觉辨识记忆和嗅球5-羟色胺能神经元的影响

目的观察嗅球注射甲苯对嗅觉功能、嗅球形态及嗅球神经生化的影响。方法甲苯1.0和3.0μL分别注入小鼠的嗅球,术后d3,5,7和14进行嗅觉辨识行为测试;术后d14免疫组织化学检测嗅球细胞5-羟色胺(5-HT)含量和5-HT能神经元数量;HE染色,显微镜下进行嗅球形态学观察。结果嗅球注射甲苯引起小鼠嗅觉记忆明显减弱甚至缺失,大剂量时难以逆转;嗅球内5-HT免疫反应阳性细胞减少;显微镜下见嗅球明显病理改变。结论甲苯对嗅觉功能产生抑制作用,嗅球细胞变性及嗅球5-HT明显减少是其原因之一。

Effects of toluene microinjecting into olfactory bulb on olfactory identification memory and serotoninergic neurons of olfactory bulb in mice

AIM To observe the effects of toluene on olfaction, morphology of cells and neurotransmitter in olfactory bulb. METHODS Toluene 1.0 and 3.0 μL was microinjected into olfactory bulb of mice. Olfactory identification memory was recorded to examine olfactory sensibility at d 3, 5, 7 and 14 after the treatments. At d 14 olfactory bulb tissues were stained with HE to observe the morphology of cells and immunohistochemistry staining was performed to observe serotoninergic neurons in olfactory bulb. RESULTS Mice in toluene groups showed decrement, even deletion of olfactory identification memory, which was unreversed in 3.0 μL group. On the d 14 after treatment with toluene, serotoninergic neurons of olfactory bulb reduced, and cell apomorphosis in olfactory bulb was observed under microscope. CONCLUSION Microinjection of toluene into olfactory bulb inhibits olfaction in mice, which maybe partially due to cell degeneration and the reduction of serotonin in olfactory bulb.