| 抗CD28抗体协同刺激增强抗CD3抗体体外激活T淋巴细胞并降低TGF-β的表达 |
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| 引用本文： | 骆群,吕明,于鸣,黎燕. 抗CD28抗体协同刺激增强抗CD3抗体体外激活T淋巴细胞并降低TGF-β的表达[J]. 中国实验血液学杂志, 2006, 14(3): 547-551 |
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| 作者姓名： | 骆群 吕明 于鸣 黎燕 |
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| 作者单位： | 1. 解放军总医院输血科,北京,100853
2. 军事医学科学院基础医学研究所免疫室,北京,100850 |
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| 摘 要： | 为了探讨体外激活T淋巴细胞的方法及活化后T淋巴细胞的免疫学特征,利用抗CD3、抗CD28抗体在体外刺激健康人外周血单个核细胞PBMNC,检测淋巴细胞转化,CD8^＋CD25^＋细胞比例和肿瘤生长因子β（TGF-β）表达等相关免疫学指标.结果表明,在抗CD28抗体的协同作用下,抗CD3抗体的刺激活性明显提高,CD8^＋CD25^＋细胞比例明显增加,TGF-β的表达下调.结论：抗CD28抗体协同刺激可以提供第二信号,使T淋巴细胞充分活化,TGF-β表达水平明显下降.
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| 关 键 词： | 抗CD3抗体 抗CD28抗体 TGF-β T细胞 |
| 文章编号： | 1009-2137（2006）03-0547-05 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-05-19 |
Anti-CD28 Antibody Costimulation Enhances Anti-CD3 antibody Activating T Cells and Lowering TGF-β Expression in Vitro |
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| LOU Qun,L Ming,YU Ming,LI Yan. Anti-CD28 Antibody Costimulation Enhances Anti-CD3 antibody Activating T Cells and Lowering TGF-β Expression in Vitro[J]. Journal of experimental hematology, 2006, 14(3): 547-551 |
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| Authors: | LOU Qun L Ming YU Ming LI Yan |
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| Affiliation: | Department of Blood Transfusion, The General Hospital of PLA, Beijing 100853, China. luoqun@public.bta.net.cn |
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| Abstract: | In order to study how to activate T cells and their immunological characteristics, the anti-CD3 and anti-CD28 McAbs were used to stimulate PBMNC, then their related immunological changes, such as lymphocyte transformation function, the percentage of CD8(+)CD25(+) cells and TGF-beta expression were deleted by lymphocyte transformation assay, flow cytometry and RT-PCR respectively. The results showed that in costimulation with anti-CD28 antibody stimulation, the activity of anti-CD3 antibody was significantly enhanced, the ratio of CD8(+)CD25(+) cells of T cells was obviously increased, while TGF-beta expression was down-regulated. It was concluded that the anti-CD28 antibody costimulation could provide stimulatory signal II, which make T cells more active, while the expression of TGF-beta significantly down-regulated. |
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