内毒素对血管内皮细胞的激活作用与动脉粥样硬化

目的：观察细菌内毒素（LPS）对人脐静脉内此细胞（HUVEC）的激活作用。探讨内皮细胞（VEC）激活在动脉粥样硬化（AS）发生发展中的意义。方法：（1）以光镜及电镜观察细胞形态。（2）测定HUVEC培养上清液中活性物质含量，以放射免疫标记技术测定内皮素（ET－1）含量，以比色法测定组织型纤溶酶原激活物（t-PA)及纤维溶酶原激 物抑制物（PAI）含量，以双抗夹心法测定因子Ⅷ相关抗原（vWF)含量。结果：（1）LPS使细胞呈长梭形，细胞收缩，间隙明显开大，细胞质水肿；（2）LPS增加培养上清液中ET－1，PAI，vWF含量（P＜0．05），对t-PA含量无明显影响（P＞0．05），结论：LPS通过影响细胞形态，促使ET－1，PAI，vWF等活性物质分泌激活VEC。激活VEC可能在AS发生，发展过程中发挥重要作用。

Activative Effect of Lipopolysaccharide Endotoxin on Vascular Endothelial Cells and Atherosclerosis

Objective\ To investigate the activative effect of lipopolysaccharide(LPS) on vascular endothelial cells(VEC) and its role in atherosclerosis.\ Methods\ The effects of LPS on morphology and secreting functions of human umbillicus vein endothelia cell(HUVEC) were observed by using following methods: cell morphology examined by light and electron\|microscopy, endothelin\|1(ET\|1) detected by radioimmunoassay, tissue\|type plasminogen activator(t\|PA) and plasminogen activator inhibitor(PAI) tested by chromatometry, and von Willebrand factor (vWF) measured by ELISA in the supernatant of medium.\ Results\ LPS caused elongation, dilatation of the intercellular junction and intracytoplasmic oedema in HUVEC.\ The activation of HUVEC with LPS resulted in higher levels of ET\|1, PAI and vWF(P<0 05).\ But LPS had no significant effects on the secretion of t\|PA in HUVEC(P>0 05).\ Conclusion\ LPS can alter vascular endothelial cell morphology and increase the secretion of ET\|1, PAI and vWF.\ The activated VEC play an important role in the formation and development of AS.