肺癌组织中端粒酶活性检测

 目的　探索端粒酶活性与肺癌发生发展的关系。方法　采用TRAP-PCR-ELISA定量及银染定性法，对30例肺手术标本进行端粒酶活 性 的分析。结果　24例肺癌组织端粒酶水平明显高于良性病变组织。端粒酶 在肺癌组织中的阳性检出率为75%。肺鳞癌端粒酶活性水平明显高于腺癌。随肿瘤范围的扩 大以及手术分期越晚的患者，其端粒酶阳性率有相对增加趋势，但差异无显著性。结论　端粒酶激活与肺癌的发生发展有密切关系。

Telomerase Activity in Human Primary Lung Cancer

Objectivc To investigate the possible relations hip between telomerase expression and progression of lung cancer. Methods The techniques of TRAP-PCR-ELISA and TRAP-silver stain i ng were employed to detecte telomerase activity in 24 fresh primary lung cance r tissues and 6 benign lung lesions obtained by surgical resection.Resul ts The levels of telomerase activity in primary lung cancer tissues was si gnificantly higher than that in benign lung lesions.Positive rate of telomerase activity detection in lung cancer patients was 75%.The level of telomerase acti v ity in squamous cell carcinomas was significantly higher than that in adenocarc i nomas.The positive rate of telomerase activity was 50% in stage Ⅱ，92.3% in st age Ⅲ.The difference between them was not significant.Conclusion Telomerase expression might contribute to the progres sion of lung cancer and could be used as a tumor marker in the diagnosis of lung cancer.