Hypermethylation of <Emphasis Type="Italic">SFRP2</Emphasis> as a Potential Marker for Stool-Based Detection of Colorectal Cancer and Precancerous Lesions |
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Authors: | Email author" target="_blank">Zhaohui?HuangEmail author Lihua?Li Jinfu?Wang |
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Institution: | (1) Oncology Institute, 4th Affiliated Hospital of Soochow University, Wuxi, 200 Huihe Road, 214062 Jiangsu Province, China;(2) College of Life Sciences, Zhejiang University, Hangzhou, China |
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Abstract: | DNA methylation is a key mechanism of colorectal carcinogenesis. Analysis of aberrantly methylation in stool DNA might provide
a novel strategy for noninvasive detection of colorectal cancer (CRC). To explore the feasibility of this approach, we have
assessed the methylation status of secreted frizzled-related protein gene 2 (SFRP2) in stool samples from patients with CRC with respect to a series of healthy individuals and patients with benign colorectal
diseases, using methylation-specific polymerase chain reaction. Methylated SFRP2 occurs in 94.2%, 52.4%, 37.5%, and 16.7% of patients with CRC, adenomas, hyperplstic polyps, and ulcerative colitis, respectively.
Of the 24 normal individuals, only 1 revealed methylated DNA. The pilot study revealed that aberrant methylated SFRP2 could be detected frequently in stools from patients with CRC and precancerous lesions. Methylation testing of fecal DNA
may be a simple, promising, and noninvasive screening tool for colorectal neoplasia. |
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Keywords: | Colorectal cancer screening SFRP2 Methylation Stool |
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