中药安迪粉针剂的放射增敏作用

目的 探讨安迪粉针剂(Andi)增强电离辐射生物学效应的作用机理。方法 以人食管癌细胞株(ECA l09)为观察对象，采用电镜、流式细胞仪分析、台盼兰染色等方法，分别观察对照、Andi、^60Co—γ线照射和Andi 照射4个组对ECA 109细胞的形态、细胞周期、细胞凋亡和坏死等的影响；并采用改良硫代巴比妥酸荧光法测定丙二醛(MDA)；羟胺法测定超氧化物歧化酶(SOD)。结果 在乏氧条件下，Andi能引起ECA 109细胞坏死(P＜0．05)，并增加MDA含量(P＜0．05)；而Andi 照射组的作用比单用Andi和单纯照射明显增强(F＝6．53，P＜0．01)；从Andi、照射、Andi照射3个组的光镜和电镜结果发现，ECA l09癌细胞明显变性、坏死、凋亡，但以Andi 照射组的改变更显著；电镜显示Andi组有较多ECA l09细胞存在糖原颗粒堆积。结论Andi可以引起乏氧ECA 109细胞的糖代谢障碍，增加细胞内自由基的产生，引起细胞坏死；Andi与^60Co—γ线合用能进一步促进乏氧细胞坏死，具有协同作用；Andi放射增敏作用的机理可能与其促进癌细胞内自由基产生或影响癌细胞糖代谢有关。

Mechanism of radiosensitizing effect of Andi

OBJECTIVE To study the mechanism of radiosensitizing effect of Andi.METHODS Human esophageal carcinoma cell line (ECA 109) were treated with culture medium, Andi, radiation and Andi combined with radiation in 99.99% nitrogen,respectively.The cells' morphological variation was observed from electroscopy. The cells'distribution in cell cycle was checked via flow cytometer. Necrosis was surveyed by dint of trypan blue stain and microscopy. Intracellular MDA and SOD were detected with modified thiobarbituric acid(TBA) fluorescence spectrophotometry of lipoperoxides (LPO) and spectrophotometry of hydroxylamine. RESULTS Under the experimental condition,necrosis of hypoxic cells and increased intracellular MDA were caused by Andi (P<0.05). With radiation to hypoxic cells, Andi produced more necrotic cells ( F=6.53,P <0.05).Under optic microscopy and electroscopy, several cells with denaturalization, necrosis or apoptosis were observed in groups treated with Andi and/or radiation. More of these changed cell were discovered in the group treated by Andi combined with radiation.Some cells with amassed hepatin were displayed by electroscopy in group treated with Andi. CONCLUSION Andi cause obstacle of glycometabolism in ECA 109 cells. It is able to aggravate cell necrosis and to increase intracellular free radical produced by radiation on the hypoxic cells.Andi with radiation further increase necrotic cells and they have cooperative effects.The mechanism of Andi's radiosensitizing effect on hypoxia cell may correlate with increasing intracellular free radical or affected glycometabolism.