| 专用采血管对血浆中循环游离DNA保存效果的影响 |
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| 引用本文： | 徐鹏,段小瑜,张海梅,原昆鹏. 专用采血管对血浆中循环游离DNA保存效果的影响[J]. 检验医学与临床, 2021, 18(8): 1035-1037,1041. DOI: 10.3969/j.issn.1672-9455.2021.08.003 |
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| 作者姓名： | 徐鹏 段小瑜 张海梅 原昆鹏 |
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| 作者单位： | 威高集团有限公司国家工程实验室,山东威海 264210;山东高创医疗器械国家研究院有限公司,山东威海 264210;山东威高集团医用高分子制品股份有限公司,山东威海 264210 |
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| 基金项目： | 国家区域创新中心科技专项项目 |
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| 摘 要： | 目的 验证专用采血管对血浆中循环游离DNA(cfDNA)保存效果的影响.方法 分别用EDTA-K2抗凝管和两种cfDNA保存专用采血管采集健康成人外周血,待血样室温保存到固定的时间点时,分离提取血浆中的cfDNA,定量检测不同时间点血浆中cfDNA水平的变化情况.结果 采集在EDTA-K2抗凝管中的血浆cfDNA水平随...
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| 关 键 词： | 专用采血管 循环游离DNA 定量检测 |
Infuence of special blood collection tubes on preservation effect of circulating free DNA in plasma |
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| XU Peng,DUAN Xiaoyu,ZHANG Haimei,YUAN Kunpeng. Infuence of special blood collection tubes on preservation effect of circulating free DNA in plasma[J]. Laboratory Medicine and Clinic, 2021, 18(8): 1035-1037,1041. DOI: 10.3969/j.issn.1672-9455.2021.08.003 |
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| Authors: | XU Peng DUAN Xiaoyu ZHANG Haimei YUAN Kunpeng |
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| Affiliation: | (National Engineering Laboratory,Weigao Group Co.,Ltd.,Weihai,Shandong 264210,China;Shandong Gaochuang Medical Device National Research Institute Co.,Ltd.,Weihai,Shandong 264210,China;Shandong Weigao Group Medical Polymer Products Co.,Ltd.,Weihai,Shandong 264210,China) |
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| Abstract: | Objective To verify the influence of special blood collection tubes(BCT)on the preservation effect of circulating free DNA(cfDNA)in plasma.Methods The peripheral blood samples in healthy adults were collected by using the EDTA-K 2 anticoagulant tubes and two kinds of special BCT for cfDNA preservation.Plasma cfDNA was isolated and extracted when the blood samples were stored at room temperature to a fixed time point.The change of plasma cfDNA level was quantitatively detected at different time points.Results The level of plasma cfDNA collected in EDTA-K 2 anticoagulant tubes was gradually increased with time lapse and then reached a stable trend,while the level of cfDNA collected in two kinds of special BCT for cfDNA preservation remained stable for at least 7 d.Conclusion The long time storage of blood samples in EDTA-K 2 anticoagulant tubes may lead plasma cfDNA level to be unstable.This change may be related to the contamination of genomic DNA in blood and the dynamic balance of the cfDNA degradation.The special BCT can stabilize the cfDNA level in plasma within at least 7 d,which plays an important role in the follow-up quantitative detection and clinical application of cfDNA. |
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| Keywords: | special blood collection tubes circulating free DNA quantitative detection |
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