基于TCGA数据库分析ANO1在结肠癌中的表达及临床意义

目的研究ANO1基因在结肠癌中的表达情况,进一步分析ANO1对结肠癌患者生存及预后的影响。方法从癌症基因组图谱(TCGA)数据库获得结肠癌mRNA表达谱数据和相应的临床信息,分析ANO1的差异表达及其与临床病理因素的相关性;进一步探索ANO1表达量对结肠癌患者预后的影响,利用基因集富集分析(GSEA)预测可能与ANO1密切相关的信号通路。结果结肠癌肿瘤组织中ANO1相对表达量明显高于癌旁组织(P<0.01);ANO1表达水平与N分期即淋巴结转移情况有关(P<0.05),与年龄、性别、肿瘤分期,以及肿瘤是否突破肌层、是否存在远处转移均无相关性(P>0.05);ANO1高表达患者总生存期明显缩短(P<0.05);Cox多因素分析可见年龄及ANO1表达状态是结肠癌患者不良预后的独立影响因素(P<0.05);ANO1高表达样本富集至钙离子信号通路、丝裂原活化蛋白激酶(MAPK)、WNT及多个癌症相关通路等基因集。结论ANO1在结肠癌肿瘤中高表达是患者不良预后的指标,且与淋巴结转移情况明显相关。ANO1有望成为结肠癌重要的预后标志物。

Analysis of the expression and clinical significance of ANO1 in colon cancer based on TCGA database

Objective To investigate the influence of ANO1 gene expression on survival and prognosis of colon cancer patients.Methods The colon cancer data set was downloaded from The Cancer Genome Atlas(TCGA)to obtain the ANO1 expression profile and clinical information.The difference of ANO1 expression and its correlation with clinical pathological factors were analyzed to elucidate the influence of ANO1 expression on the prognosis of patients with colon cancer.Gene set enrichment analysis(GSEA)was used to predict the regulatory signaling pathways of ANO1.Results The expression level of ANO1 mRNA in tumor tissues of colon cancer was significantly higher than in para-carcinoma tissues(P<0.01).N stage,also referred to lymph node metastasis correlated with ANO1 expression level(P<0.05),while age,gender,tumor stage,muscularis breakthrough and distant metastasis were not correlated(P>0.05).The overall survival of patients with high ANO1 expression significantly shortened(P<0.05).Cox multivariate analysis showed that age and ANO1 expression status were the independent factor affecting poor prognosis in colon cancer patients(P<0.05).GSEA showed that high expression samples of ANO1 were enriched to such gene sets as calcium ion signaling pathway,MAPK,WNT and multiple cancer-related pathways.Conclusion ANO1 significantly up-regulated in colon cancer and correlated with lymph node metastasis.Its high expression is an indicator of poor prognosis in patients.ANO1 could be a potential prognostic marker of colon cancer.