酶联免疫吸附试验与化学发光法检测TORCH抗体的方法学对比研究

目的 探讨酶联免疫吸附试验(ELISA)与化学发光法(CLIA)分别检测TORCH抗体的价值.方法 将2017年2月至2019年7月在该院进行孕前或孕期的致畸五项(TORCH抗体)检查的632例孕妇纳入研究,采集静脉血标本进行ELISA、CLIA检测,并采用病原体培养分离鉴定结果作为金标准,以此对比两种检查方法的阳性率...

Methodologic comparative study on enzyme-linked immunosorbent assay and chemiluminescent immunoassay for detecting TORCH antibody

Objective To explore the value of enzyme-linked immunosorbent assay(ELISA)and chemiluminescent immunoassay(CLIA)in detecting TORCH antibodies.Methods A total of 632 venous blood samples from the women undergoing prenatal or gestational TOX-Other-RV-CMV-HSV(TORCH)antibody examinations in this hospital from February 2017 to July 2019 were enrolled in the study and examined by ELISA and CLIA.By taking the identification results of pathogen culture and separation as the golden standard,the positive rate,sensitivity,specificity,accuracy,positive predictive value and negative predictive value were compared between the two methods.Results The positive rates of TOX,RV,CMV,HSVⅠand HSVⅡantibodies detected by ELISA were significantly lower than those by CLIA,and the differences were statistically significant(P<0.05).The sensitivity and negative predictive value of CLIA for detecting TORCH antibody were significantly higher than those of ELISA(98.46%,99.59%vs.60.00%,90.55%),and the differences were statistically significant(P<0.05).There was no statistically significant difference in the specificity,accuracy and positive predictive value between CLIA and ELISA(96.41%,96.84%,87.67%vs.99.20%,91.14%,95.12%,P>0.05).Conclusion ELISA and CLIA for detecting TORCH antibodies have no obvious differences in the accuracy,specificity and positive predictive value and can serve as the effective means for clinical diagnosis.CLIA is superior in terms of positive rate,sensitivity and negative predictive value.