脑胶质瘤中磷脂酰肌醇3-激酶、蛋白激酶B以及同源磷酸酶-张力蛋白表达水平

目的 探讨PI3K、AKT、PTEN 在脑胶质瘤中的表达。方法 选择2017 年1~12 月在我院及华山医院手术治疗的脑胶质瘤患者66 例的病理标本进行分析，另选择50 例患者的正常脑组织病理标本为参照。应用免疫组化方法检测磷脂酰肌醇3-激酶、蛋白激酶B 及同源磷酸酶-张力蛋白表达，比较脑胶质瘤组织中以及正常脑组织中三者的表达水平，分析脑胶质瘤组织中三者阳性表达的相关性。结果 PI3K 及AKT 在正常脑组织的阳性表达率显著低于低级别脑胶质瘤及高级别脑胶质瘤，差异有统计学意义（P<0.05）；低级别脑胶质瘤显著低于高级别脑胶质瘤，差异有统计学意义（P<0.05）。正常脑组织PTEN 蛋白阳性率100.0%，显著高于低级别脑胶质瘤与高级别脑胶质瘤，差异有统计学意义（P<0.05）；低级别脑胶质瘤显著高于高级别脑胶质瘤，差异有统计学意义（P<0.05）。结论 PI3K、AKT 在脑胶质瘤中呈高表达，PTEN 呈低表达，且与恶性程度有关。

The expression levels of phosphatidylinositol 3 -kinase, protein kinase B and phosphatase and tensin homolog in gliomas

Objective To investigate the expression of PI3K, AKT and PTEN in gliomas. Methods The pathological specimens of 66 glioma patients who underwent surgery in our hospital and Huashan Hospital from January to December 2017 were analyzed, and another 50 normal pathological specimens were selected as reference group. Immunohistochemistry was used to detect the expressions of phosphatidylinositol 3-kinase, protein kinase B and homologous phosphatase-tensin. The expression of three indicators between glioma tissues and normal brain tissues was compared to analyze the correlation between the three positive expressions in glioma tissues. Results The positive expression rates of PI3K and AKT in normal brain tissue were significantly lower than those in low-grade gliomas and high-grade gliomas,and the difference was significant (P<0.05). And the positive expression rates of PI3K and AKT in the low-grade gliomas were significantly lower than those in high-grade gliomas, and the difference was statistically significant (P<0.05). The positive rate of PTEN protein in normal brain tissue was 100.0%, which was significantly higher than that in low-grade glioma and high-grade glioma, with significant difference (P<0.05). And the positive rate of PTEN protein in the low malignant glioma was significantly higher than that in high grade glioma, and the difference was statistically significant (P<0.05). Conclusion PI3K and AKT are highly expressed and PTEN is low-expressed in gliomas, which is related to the degree of malignancy.