男性不育患者精子DFI与其他精液参数的关系

目的通过研究精子DNA碎片指数(DFI)与其他精液参数的相关性,探讨精子DFI在诊断和治疗男性不育中的临床应用价值。方法收集2019年1月至2020年1月在该院生殖中心就诊的959例男性不育患者的精液标本,通过计算机辅助分析系统进行精液常规检测,运用Diff-quik染色法对精液进行染色处理后再作形态学分析,采用精子染色质扩散试验检测精子DFI,根据精子DFI值分组,Ⅰ组精子DFI<30%,Ⅱ组精子DFI≥30%,并对精子DFI与其他精液参数的相关性进行分析。结果Ⅰ组有754例;Ⅱ组有205例。Ⅰ组的精子存活率、前向运动精子百分率(PR)、非前向运动精子百分率(NP)和正常形态精子百分率明显高于Ⅱ组,差异有统计学意义(P<0.05)。Ⅰ组患者的年龄、不动精子百分率(IM)明显低于Ⅱ组,差异有统计学意义(P<0.05)。精子DFI与精子存活率、PR、NP、正常形态精子百分率呈负相关(r=-0.409、-0.402、-0.198、-0.216,P<0.05),而与患者年龄和IM呈正相关(r=0.181、0.402,P<0.05)。结论精子DFI与多项精液参数相关,因此精子DFI检测应与精液常规检测及精子形态学分析相结合,为临床评估男性不育患者的生育力提供可靠且准确的评价指标。

Correlation between sperm DF1 and other semen parameters of male infertility patients

Objective To investigate the clinical application value of sperm DNA fragmentation index(DFI)in the diagnosis and treatment of male infertility diseases by studying the correlation between sperm DFI and other semen parameters.Methods Collect semen specimens of 959 male infertile patients who were treated in Center for Reproductive Medicine of the Sixth Affiliated Hospital of Guangzhou Medical University/Qingyuan People′s Hospital from January 2019 to January 2020,detect semen parameters by computer-aided analysis system,and use Diff-quik staining to stain the semen,morphological analysis was performed afterwards,and sperm DFI was detected by SCD method,and grouped as groupⅠwith DFI<30%,groupⅡwith DFI≥30%according to sperm DFI value,and the correlations between sperm DFI and other semen parameters were analyzed.Results GroupⅠhad 754 cases,groupⅡhad 205 cases.Sperm survival rate,forward movement of the sperm percentage(PR),non forward movement sperm percentage(NP)and normal morphology sperm percentage in groupⅠwere higher than those in groupⅡ,the differences were statistically significant(P<0.05).Patients′age,motionless sperm percentage(IM)in groupⅠwere lower than those in groupⅡ,the differences were statistically significant(P<0.05).Correlation analysis found that sperm DFI value significantly negatively correlated with sperm survival rate,PR,NP and normal morphology sperm percentage(r=-0.409,-0.402,-0.198,-0.216,P<0.05),and positively related to the patients′age,IM(r=0.181,0.402,P<0.05).Conclusion Sperm DFI significantly relates to other semen parameters.Therefore,sperm DFI testing should be combined with routine semen analysis and morphological analysis to provide a reliable and accurate evaluation index for clinically evaluating the fertility of male infertile patients.