人类白细胞抗原DRB1等位基因与幽门螺杆菌感染的关系

目的 研究人类白细胞抗原（ＨＬＡ）ＤＲＢ１等位基因与幽门螺杆菌（Ｈｐ）感染的关系。方法 用Ｂｉｏｓｅｅｄ Ｈｐ－ＩｇＧ定量酶联免疫试剂盒检测４６例胃癌、７５例食道癌和１００例人群对照的Ｈｐ－ＩｇＧ抗体，用Ｂｉｏｔｅｓｔ低解析水平ＨＬＡ－ＤＲＢ酶联免疫探针杂交测定试剂盒检测ＨＬＡ－ＤＲＢ１等位基因。结果 （１）Ｈｐ－ＩｇＧ阳性组ＤＲＢ１＊０８基因频率显著高于Ｈｐ－ＩｇＧ阴性组（１３．１％ｖｓ４．４％

Study on the relations between HLA-DRB1 alleles and Helicobacter pyloriinfection

OBJECTIVE: In order to study the relation between human leukocyte antigen (HLA) DRB1 alleles and Helicobacter pylori (Hp) infection. METHODS: Hp-IgG antibody from 46 gastric cancer (GC), 75 esophageal cancer and 100 population-based controls were identified by Hp-IgG quantitative enzyme immunoassay. Biotest HLA-DRB enzyme linked probe hybridization assay kit (low resolution) was used to identify DRB1 alleles. RESULTS: (1) Frequency of DRB1 * 08 was significantly higher in Hp-IgG positive group than in Hp-IgG negative group (13.1% vs 4.4%, chi(2) = 11.14, P < 0.001). Frequency of DRB1 * 12 was significantly lower in Hp-IgG positive group than in Hp-IgG negatives (5.4% vs 11.3%, chi(2) = 4.49, P < 0.05). (2) Frequency of DRB1 * 02 in GC was significantly higher than that of controls. Frequency of DRB1 * 07 in GC was significantly lower than that of controls. However, neither the frequency of DRB1 * 02 between Hp-IgG positive and Hp-IgG negative groups nor the frequency of DRB1 * 07 between Hp-IgG positive and Hp-IgG negative groups showed significant differences in GC and controls. CONCLUSIONS: (1) HLA-DRB1 * 08 might serve a genetic risk factor for Hp infection while DRB1 * 12 might play a role of protecting effect against Hp infection. (2) DRB1 * 02 might be a genetic risk factor for GC while DRB1 * 07 might play a role of protecting effect against GC. However, the relations between DRB1 * 02, DRB1 * 07 and GC were not associated with Hp infection.