|
|||||
|
|
| 一步亲和纯化制备基因工程人源抗HBs单克隆抗体Fab片段 | |
| 引用本文： | 胡栋平,韩焕兴,尤长宣,罗荣城,毛庆民. 一步亲和纯化制备基因工程人源抗HBs单克隆抗体Fab片段[J]. 第二军医大学学报, 2000, 21(1): 50-52 |
| 作者姓名： | 胡栋平 韩焕兴 尤长宣 罗荣城 毛庆民 |
| 作者单位： | 1. 第二军医大学长征医院临床免疫中心,上海,200003 2. 广州第一军医大学南方医院生物治疗中心 3. 济南军区第107医院检验科 |
| 基金项目： | 国家自然科学基金!资助项目（39670668） |
| 摘 要： | 目的：探索亲和纯化人源单克隆抗体片段的简便实用方法。方法：应用自制的抗人免疫球蛋白片段抗体（Ａｎｔｉ－Ｆａｂ）与链球菌蛋白Ｇ亲和胶（Ｇａｍｍａ Ｂｉｎｄ）交联制备亲和层析柱,用一步法从工程菌培养上清中纯化人源抗ＨＢｓ单克隆Ｆａｂ片段。经依沙吖啶沉淀、离子交换、分子筛纯化人混合血清提取ＩｇＧ组分,木瓜酶处理后分离,纯化出Ｆａｂ,免疫绵羊制备高效价抗表。用Ｇａｍｍａ Ｂｉｎｄ一步纯化出抗血清中的ＩｇＧ |
| 关 键 词： | 乙型肝炎 抗HBs 单克隆抗体 Fab片段 |
| 文章编号： | 0258-879X(2000)01-0050-03 |
| 修稿时间： | 1999-08-30 |
Affinity purification of engineered human monoclonal antibody HBs Fab fragments |
|
| HU Dong-Ping,HAN Huan-Xing,YOU Chang-Xuan,LUO Rong-Cheng,MAO Qing-Min. Affinity purification of engineered human monoclonal antibody HBs Fab fragments[J]. Former Academic Journal of Second Military Medical University, 2000, 21(1): 50-52 | |
| Authors: | HU Dong-Ping HAN Huan-Xing YOU Chang-Xuan LUO Rong-Cheng MAO Qing-Min |
| Affiliation: | HU Dong-Ping(Clinical Immunology Center, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)HAN Huan-Xing(Clinical Immunology Center, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)YOU Chang-Xuan(Biological Treatment Center, Nanfang Hospital, First Military Medical University)LUO Rong-Cheng(Biological Treatment Center, Nanfang Hospital, First Military Medical University)MAO Qing-Min(Department of Clinical Laboratories, Hospital No. 107, Jinan Military Area Command) |
| Abstract: | |
| Keywords: | antibodies monoclone affinity purification hepatitis B hepatitis antibodies |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |