胡芦巴种子有效成分对实验性糖尿病大鼠肾脏病变的保护作用

目的：探讨胡芦巴种子A、B成分对实验性糖尿病大鼠肾脏病变的保护作用。方法：利用链脲佐菌素(STZ)制作糖尿病大鼠模型，随机分为:糖尿病组(D)8只,胡芦巴种子A成分治疗组(A)10只, 胡芦巴种子B成分治疗组(B)10只，另设正常对照组（C）6只。用胡芦巴种子A、B成分灌胃糖尿病大鼠12周后，观察其对血糖、血脂水平、肾功能、肾脏指数、肾小球体积及肾脏病变的影响。结果：实验性糖尿病大鼠应用胡芦巴种子A、B成分治疗后，A、B组血糖，尿蛋白排泄率、血肌酐和尿素氮、肾指数和肾小球体积均明显低于Ｄ组(P<0.05，P<0.01)。A组大鼠甘油三酯(TG)、低密度脂蛋白(LDLC)比D组明显降低(P<0.05)，高密度脂蛋白(HDLC)较D组明显升高(P<0.01)，而胆固醇(CHO)无明显改变(P>0.05)；B药治疗组大鼠TG、LDLC及CHO均明显降低，而HDLC水平则明显升高，与D组比较差异有显著性(P<0.01)。光电镜结果显示，A、B两组肾小球病变均轻于D组，且B组肾小球结构稍强于A组。结论：胡芦巴种子A、B成分能够调节实验性糖尿病大鼠糖、脂代谢，改善肾功能，减轻肾脏病变程度，对实验性糖尿病大鼠肾脏病变具有一定的保护作用，B成分的作用强于A成分。

Protective effects of fenugreek seeds active components on renal lesion in experimental diabetic rats

Objective To study the protective effects of fenugreek seeds components A,B on renal lesion in diabetic rats.Methods The model of diabetes mellitus was built with streptozotocin(STZ) in rats.All the rats were randomly divided into four groups: diabetes control group(D)(n=8),fenugreek seeds components A treatment group(A)(n=10),fenugreek seeds components B treatment group(B)(n=10),and normal control group(C)((n=6).) Diabetic rats were treated with fenugreek seeds components A,B for 12 weeks,The metabolism levels of blood glucose,blood lipids were determined.Kidney morphology was observed to evaluate the protective effects of fenugreek seeds components A,B on renal structure and function in diabetic rats.Results In diabetic rats treated with fenugreek seeds components A and B,the levels of blood glucose,urinary protein excretion,serum creatinine,blood urea nitrogen,kidney index and glomerular volume were significantly lower than those in group D((P>0.05,)P<0.01).In group A,the levels of TG,LDLC were lighter than those in group D((P>0.05),)HDLC was higher than that in group D((P<0.01),)CHO had no significant difference with group D.In group B,the levels of TG,LDLC and CHO were lighter than those in group D,whereas the level of HDLC was higher than that in group D((P<0.01)).Optical and electro microscopic findings indicated that the lesions of glomerular of rats in group B were alleviated significantly,and the glomerular structure in group B was better than that in group A.(Conclusion Fenugreek) seeds components A,B can modulate the metabolism of blood glucose,blood lipids in diabetic rats.Functional and morphological abnormalities in kidney have been lightened.Fenugreek seeds components may protect the kidney of diabetic rats to a certain degree.The protective effect of components B is better than that of components A.