缺血后处理对大鼠心肌缺血再灌注时血清TNF-α、IL-1β和IL-6浓度的影响

目的:本研究旨在观察缺血后处理对心脏缺血再灌注中大鼠心肌组织炎性细胞因子的影响,并探讨可能的保护机制。方法:30只雄性SD大鼠随机分成3组:假手术组(A组,n=10),缺血再灌注组(B组,n=10),缺血后处理组(C组,n=10),结扎左冠状动脉制造心脏缺血再灌注模型,监测围手术期血流动力学变化,收集手术后心肌组织,计算其心肌梗死面积,采集开胸前(T0),缺血再灌注损伤后(T1),手术结束时(T2)的新鲜血,观察肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1),白介素-6(IL-6)的变化,探讨缺血后处理对心肌缺血再灌注损伤的保护作用。结果:与B组相比,C组围手术期的血流动力学变化不明显(P<0.05),心肌梗死面积减少(P<0.05),C组中的TNF-α,IL-1,IL-6在相同时点的含量降低(P<0.05)。结论:缺血后处理对I/R损伤有显著的保护作用,机理可能与其抑制炎性细胞因子TNF-α,IL-1,IL-6的合成和释放,从而减轻中性粒细胞的浸润与激活有关。

Effect of Ischemic Postconditioning on Cytokines After Acute Ischemic Injury

Objective: To observe the effect of ischemic postconditioning on serum cytokines after acute ischemic reperfusion injury in rats.Methods: Thirty male SD rats were randomly devided into sham-operated group(group A,n=10),ischemia-reperfusion injury group(group B,n=10) and ischemic postconditioning group(group C,n=10).The haemodynamics changes around operation were monitored.Myocardial infarct area was calculated,and blood samples were collected before opening the chest(T0),after ishchemic reperfusion injury(T1) and after operation(T2) for observing the changes of TNF-α,IL-1 and IL-6.Results: Compared with that in group B,the haemodynamic change aroud operation of group C was not significant(P<0.05),but the myocardial infarct area and contents of TNF-α,IL-1 and IL-6 were decreased(all P<0.05).Conclusion: Ischemic postconditioning might protect myocardium from ischemic reperfusion injury through inhibiting the serum levels of TNF-α,IL-1 and IL-6.