胍丁胺对新生大鼠海马神经前体细胞增殖的影响

目的探讨胍丁胺对大鼠海马神经前体细胞增殖的影响及可能的作用机制。方法分离新生24 h大鼠的海马组织,制成单细胞悬液后进行无血清条件培养;用CCK-8试剂盒和3H-胸腺嘧啶核苷参入法检测细胞增殖和胍丁胺的作用。结果从新生大鼠海马分离得到的细胞可以持续分裂增殖形成细胞克隆(神经球);胍丁胺1、10μmol.L-1可以显著促进神经前体细胞的增殖,并且这种促增殖作用可以被依法克生所抑制。结论胍丁胺可以促进体外培养的新生大鼠海马神经前体细胞的增殖,其促增殖作用可能与咪唑啉Ⅰ(I1)受体有关。

Effects of agmatine on the proliferation of progenitor cells from neonatal rat hippocampus

Aim To study the effects of agmatine on the proliferation ability of neural progenitor cells from neonatal rat hippocampus.Methods Hippocampus of neonatal rat was isolated and made into single-cell suspension,which was cultured in serum-free medium and then using CCK-8 kit assay,the survival rate of neurospheres incubated with various concentrations of agmatine and efaroxan.Furthermore,the effect of these drugs was measured with()~3H-thymidine incorporation assay.Results Cells we cultured could continuously proliferate and cultured as floating neurospheres.CCK-8 kit assay revealed that agmatine 1 μmol·L~(-1) and 10 μmol·L~(-1) enhanced the survival rate of neural stem cell,and the result alike with()~3H-thymidine incorporation assay.Efaroxan 10 μmol·L~(-1) inhibit proliferation effect of agmatine on neural stem cell.Conclusion Agmatine was found to increase the proliferation of neural stem cell and efaroxan can block the proliferation effect.It suggested that imidazolineⅠ receptor may be related to the proliferation effect.